Mitchell P J, Timmons P M, Hébert J M, Rigby P W, Tjian R
Howard Hughes Medical Institute, Department of Molecular and Cellular Biology, University of California, Berkeley 94720.
Genes Dev. 1991 Jan;5(1):105-19. doi: 10.1101/gad.5.1.105.
We have analyzed the expression pattern of transcription factor AP-2 in mouse embryos to evaluate the potential of AP-2 as a regulator during vertebrate development. A partial cDNA encoding AP-2 was isolated from a mouse embryo cDNA library and used to prepare probes to measure AP-2 mRNA levels by RNase protection and RNA in situ hybridization. Between 10.5 and 15.5 days of embryogenesis, the relative abundance of AP-2 mRNA is greatest at 11.5 days and declines steadily thereafter. RNA in situ hybridization analysis of embryos between 8.5 and 12.5 days of gestation identified a novel expression pattern for AP-2. The principle part of this expression occurs in neural crest cells and their major derivatives, including cranial and spinal sensory ganglia and facial mesenchyme. AP-2 is also expressed in surface ectoderm and in a longitudinal column of the spinal cord and hindbrain that is contacted by neural crest-derived sensory ganglia. Additional expression of AP-2 occurs in limb bud mesenchyme and in meso-metanephric regions. This embryonic expression pattern is spatially and temporally consistent with a role for AP-2 in regulating transcription of genes involved in the morphogenesis of the peripheral nervous system, face, limbs, skin, and nephric tissues.
我们分析了转录因子AP-2在小鼠胚胎中的表达模式，以评估AP-2在脊椎动物发育过程中作为调节因子的潜力。从一个小鼠胚胎cDNA文库中分离出编码AP-2的部分cDNA，并用于制备探针，通过核糖核酸酶保护法和RNA原位杂交来测量AP-2 mRNA水平。在胚胎发育的10.5至15.5天之间，AP-2 mRNA的相对丰度在11.5天时最高，此后稳步下降。对妊娠8.5至12.5天的胚胎进行RNA原位杂交分析，确定了AP-2的一种新的表达模式。这种表达的主要部分发生在神经嵴细胞及其主要衍生物中，包括颅和脊髓感觉神经节以及面部间充质。AP-2也在表面外胚层以及脊髓和后脑的一个纵向柱中表达，该纵向柱与神经嵴衍生的感觉神经节接触。AP-2在肢芽间充质和中后肾区域也有额外表达。这种胚胎表达模式在空间和时间上与AP-2在调节参与外周神经系统、面部、四肢、皮肤和肾组织形态发生的基因转录中的作用一致。
