State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
Macromol Biosci. 2009 Dec 8;9(12):1202-10. doi: 10.1002/mabi.200900208.
A temperature-sensitive block copolymer (PAAm-b-PNIPAAm-Te) with a glutathione peroxidase-like active site was synthesized via ATRP. As a new glutathione peroxidase (GPx) mimic, it displays typical saturation kinetic behaviors and high catalytic activity. More importantly, the catalytic activity of the polymer can be well modulated by changing the temperature. The experiments proved that a change in the self-assembly structure of the polymer plays a key role for the modulation of catalytic activity. As a contrast, another block copolymer, PAAm-Te-b-PNIPAAm, was synthesized. In comparison with PAAm-b-PNIPAAm-Te, the different temperature dependent catalytic behavior seen further indicates that the micellar structure plays an important role in modulating the catalytic activity of the smart enzyme model.
通过原子转移自由基聚合（ATRP）合成了一种具有谷胱甘肽过氧化物酶样活性位点的温敏嵌段共聚物（PAAm-b-PNIPAAm-Te）。作为一种新型谷胱甘肽过氧化物酶（GPx）模拟物，它表现出典型的饱和动力学行为和高催化活性。更重要的是，通过改变温度可以很好地调节聚合物的催化活性。实验证明，聚合物自组装结构的变化对于调节催化活性起着关键作用。相比之下，还合成了另一种嵌段共聚物 PAAm-Te-b-PNIPAAm。与 PAAm-b-PNIPAAm-Te 相比，所观察到的不同温度依赖性催化行为进一步表明胶束结构在调节智能酶模型的催化活性方面起着重要作用。
