Thobunluepop Pitipong
Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Talard Sub-District, Muang District, Maha Sarakham 44000, Thailand.
Pak J Biol Sci. 2009 Aug 15;12(16):1102-10. doi: 10.3923/pjbs.2009.1102.1110.
Presently, chemical seed treatments are in discussion due to their directly or indirectly impacts on human health or other living organisms. They may also negatively affect the ecosystem and the food chain. In rice seeds, chemicals may cause phytotoxic effects including seed degradation. Eugenol is the main component of clove (Eugenia caryophillis) oil, which was proved to act simultaneously as bactericide, virocide and especially fungicide. The in vitro study was aimed to compare the inhibitory effect of the following seed treatment substances against seed borne fungi and their shelf-life during 12 months of storage; conventional captan (CA), chitosan-lignosulphonate polymer (CL), eugenol incorporated into chitosan-lignosulphonate polymer (E+CL) and control (CO). The obtained results of fungi inhibition were classified in three groups, which showed at first that CA treatment led to a better, i.e., longer, inhibitory effect on Alternaria padwickii, Rhizoctonia solani, Curvularia sp., Aspergillus flavus and Aspergillus niger than E+CL. Secondly, E+CL coating polymer showed the longest inhibitory effect against Bipolaris oryzae and Nigrospora oryzae compared to CA and CL coating polymer. Finally, both CA and E+CL coating polymer had non-significant difference inhibitory effect on Fusarium moniliforme. The variant of CL coating polymer for seed coating was only during the first 6 months of storage able to inhibit all species of the observed seed borne fungi, whereas CA and E+CL coating polymer were capable to inhibit most of the fungi until 9 months of storage.
目前，由于化学种子处理对人类健康或其他生物体有直接或间接影响，因此正在对其进行讨论。它们还可能对生态系统和食物链产生负面影响。在水稻种子中，化学物质可能会导致植物毒性效应，包括种子降解。丁香酚是丁香（ Eugenia caryophillis）油的主要成分，已被证明同时具有杀菌、杀病毒尤其是杀真菌的作用。体外研究旨在比较以下种子处理物质对种子携带真菌的抑制作用及其在储存12个月期间的保质期；传统的克菌丹（CA）、壳聚糖-木质素磺酸盐聚合物（CL）、掺入壳聚糖-木质素磺酸盐聚合物的丁香酚（E+CL）和对照（CO）。所获得的真菌抑制结果分为三组，首先表明CA处理对稻梨孢链格孢、立枯丝核菌、弯孢属、黄曲霉和黑曲霉的抑制作用更好，即更长，优于E+CL。其次，与CA和CL涂层聚合物相比，E+CL涂层聚合物对稻瘟病菌和稻黑孢霉的抑制作用持续时间最长。最后，CA和E+CL涂层聚合物对串珠镰刀菌的抑制作用无显著差异。用于种子包衣的CL涂层聚合物变体仅在储存的前6个月能够抑制所有观察到的种子携带真菌物种，而CA和E+CL涂层聚合物能够在储存9个月之前抑制大多数真菌。
