Ito M, Kaneko-Ishino T, Ishino F, Matsuhashi M, Yokoyama M, Katsuki M
Institute of Applied Microbiology, University of Tokyo, Japan.
J Exp Zool. 1991 Feb;257(2):178-83. doi: 10.1002/jez.1402570206.
The developmental capability of haploid parthenogenetic cells was investigated by studies on haploid parthenogenetic in equilibrium fertilized mouse chimeras. Two chimeras were born. One female chimera was smaller at birth and grew slower than its littermates. The distribution of haploid-derived cells in the chimeras was analyzed 11 months after their birth. Cells derived from haploid embryos were found only in the brain, eyes, pigment cells in hair follicles, and spleen, in which they constituted 30%, 20%, 10%, and less than 5%, respectively, of the cells. The correlation between the parthenogenetic contribution to the brain and growth retardation is discussed. All of the cells examined in these chimeric organs (brain and eyes) contained a diploid amount of DNA, suggesting that diploidization of the haploid parthenogenetic cells occurred during development. Possibly, the haploid state is not sufficient for cell growth, even in chimeras with fertilized embryos.
通过对单倍体孤雌生殖与平衡受精的小鼠嵌合体的研究，对单倍体孤雌生殖细胞的发育能力进行了调查。诞生了两只嵌合体。一只雌性嵌合体出生时体型较小，生长速度比其同窝仔慢。在它们出生11个月后，分析了嵌合体中源自单倍体的细胞分布。仅在脑、眼、毛囊色素细胞和脾脏中发现了源自单倍体胚胎的细胞，在这些器官中，它们分别占细胞总数的30%、20%、10%和不到5%。讨论了孤雌生殖对脑的贡献与生长迟缓之间的相关性。在这些嵌合器官（脑和眼）中检查的所有细胞都含有二倍体数量的DNA，这表明单倍体孤雌生殖细胞在发育过程中发生了二倍体化。可能即使在与受精胚胎的嵌合体中，单倍体状态也不足以支持细胞生长。
