VPg介导的马铃薯Y病毒属病毒RNA聚集

VPg-mediated aggregation of potyviral RNA.

作者信息

Luciano C S, Murphy J F, Rhoads R E, Shaw J G

机构信息

Department of Plant Pathology, University of Kentucky, Lexington 40546.

出版信息

J Gen Virol. 1991 Jan;72 ( Pt 1):205-8. doi: 10.1099/0022-1317-72-1-205.

DOI:10.1099/0022-1317-72-1-205

PMID:1990064

Abstract

RNA prepared from the potyvirus tobacco vein mottling virus contained aggregates of the 9.5 kb genomic RNA with electrophoretic mobilities corresponding to 20 and 41 kb species. Similar aggregates were present in preparations of the RNAs of two other potyviruses. Aggregation occurred during or after purification of the RNA by sucrose gradient centrifugation and alcohol precipitation and was dependent upon the presence of a protein apparently bound covalently to a region at or near the 5' terminus of the viral RNA. This protein is probably the VPg. The RNAs of tobacco mosaic virus and cowpea mosaic virus did not form aggregates when isolated from purified virus by similar procedures.

摘要

从马铃薯Y病毒属烟草脉斑驳病毒制备的RNA含有9.5 kb基因组RNA的聚集体，其电泳迁移率对应于20 kb和41 kb的条带。另外两种马铃薯Y病毒的RNA制剂中也存在类似的聚集体。聚集体在通过蔗糖梯度离心和乙醇沉淀纯化RNA的过程中或之后形成，并且依赖于一种显然与病毒RNA 5'末端或其附近区域共价结合的蛋白质的存在。这种蛋白质可能是病毒基因组连接蛋白（VPg）。当通过类似方法从纯化病毒中分离时，烟草花叶病毒和豇豆花叶病毒的RNA不会形成聚集体。