B' Department of Neurology, AHEPA University Hospital, Thessaloniki, Greece.
Lab Anim. 2010 Apr;44(2):71-8. doi: 10.1258/la.2009.009043. Epub 2009 Nov 9.
Intracerebroventricular (ICV) route of administration is a useful experimental method to study the effects of chemicals or cellular grafts in the ventricular compartment of the brain after focal ischaemia. However, the induced oedema may cause structural dislocating phenomena and render a stereotaxic ICV invasion difficult and practically unavailable especially during the acute post-ischaemia phase. The aim of this study was to measure these structural ventricular dislocations and set new stereotaxic coordinates for successful and cost-effective ICV invasion 6-18 h after focal cerebral ischaemia. Wistar rats were subjected to 2 h middle cerebral artery occlussion (t-MCAO), were neurologically evaluated (modified Neurological Stroke Scale [mNSS], modified Bederson's Scale [mBS] and grid-walking test [GWT]) and brain slides were studied at 6 and 18 h post-occlusion for infarction volume, hemispheric oedema, middle line dislocation and stereotaxia of the lateral ventricles. Our data indicated that stereotaxic coordinates of the lateral ventricles in the infarcted and contralateral hemispheres significantly (P < 0.05) changed at both time-points and were linearly correlated with the mNSS, mBS and some GWT scores (P < 0.001). This correlation allowed for the calculation of simple (linear) mathematical equations (stereotaxic coordinate = b0 + b1*mNSS, where 'b0' and 'b1' are fixed number and factor, respectively, calculated by regression analysis) that determined individually new coordinates for each animal. Verification experiments revealed that the new coordinates render ICV invasion feasible in up to 80% of infarcted rats (number needed to treat 1.65), compared with only 19.4% using the classical coordinates for normal rats. Therefore, we propose a new, time- and cost-effective methodology for practically feasible ICV invasion in rats 6-18 h after t-MCAO.
脑室内（ICV）给药途径是一种有用的实验方法，可用于研究局灶性缺血后大脑室腔内化学物质或细胞移植物的作用。然而，诱导的脑水肿可能导致结构移位现象，使立体定向 ICV 入侵变得困难且实际上不可用，尤其是在急性缺血后阶段。本研究旨在测量这些结构性脑室移位，并为成功和具有成本效益的 ICV 入侵设定新的立体定向坐标，即在局灶性脑缺血后 6-18 小时。Wistar 大鼠接受 2 小时大脑中动脉闭塞（t-MCAO），进行神经学评估（改良神经中风量表 [mNSS]、改良贝德森量表 [mBS] 和网格行走测试 [GWT]），并在闭塞后 6 和 18 小时对脑切片进行梗死体积、半球水肿、中线移位和侧脑室立体定向研究。我们的数据表明，在两个时间点，梗塞和对侧半球的侧脑室立体定向坐标都显著（P < 0.05）发生变化，并且与 mNSS、mBS 和一些 GWT 评分呈线性相关（P < 0.001）。这种相关性允许计算简单的（线性）数学方程（立体定向坐标=b0+b1*mNSS，其中“b0”和“b1”分别是通过回归分析计算的固定数字和因子），这些方程分别为每个动物确定新的坐标。验证实验表明，与使用经典坐标对正常大鼠进行 ICV 入侵的成功率（19.4%）相比，新坐标可使多达 80%的梗塞大鼠的 ICV 入侵成为可能（需要治疗的人数 1.65）。因此，我们提出了一种新的、时间和成本有效的方法，可在 t-MCAO 后 6-18 小时内实际可行地进行大鼠的 ICV 入侵。
