Yang Liu, Kim Joon-Hyun, Kovacs Kyle D, Arroyo Jorge G, Chen Dong F
Department of Ophthalmology, Peking University First Hospital, Beijing, China.
Arch Ophthalmol. 2009 Nov;127(11):1475-80. doi: 10.1001/archophthalmol.2009.288.
To determine whether systemic minocycline can protect photoreceptors in experimental retinal detachment (RD).
Retinal detachment was induced in mice by subretinal injection of sodium hyaluronate, 1.4%. In 1 experiment, mice received daily injections of minocycline (group 1) or saline (group 2). In a second experiment, mice were treated with minocycline or saline beginning 24 hours prior, immediately after, or 24 hours after experimental RD. In both experiments, photoreceptor cell survival and apoptosis were assessed by immunohistochemistry with primary antibodies against photoreceptor cell markers, rod rhodopsin, and cone opsin, and by terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling.
Photoreceptor cell apoptosis was detected at day 1 after experimental RD, with apoptotic cells peaking in number at day 3 and dropping by day 7. Treatment with minocycline significantly reduced the number of apoptotic photoreceptor cells associated with RD when given 24 hours before or even 24 hours after RD.
Our data suggest that minocycline may be useful in the treatment of photoreceptor degeneration associated with RD, even when given up to 24 hours after RD.
Use of minocycline in patients with macula-off RD may prevent photoreceptor apoptosis and glial cell proliferation, improving final visual outcomes.
确定全身性米诺环素是否能在实验性视网膜脱离（RD）中保护光感受器。
通过视网膜下注射1.4%的透明质酸钠在小鼠中诱导视网膜脱离。在1个实验中，小鼠每日注射米诺环素（第1组）或生理盐水（第2组）。在第2个实验中，小鼠在实验性视网膜脱离前24小时、脱离后立即或脱离后24小时开始用米诺环素或生理盐水治疗。在这两个实验中，通过使用针对光感受器细胞标志物、视杆细胞视紫红质和视锥细胞视蛋白的一抗进行免疫组织化学，以及通过末端脱氧核苷酸转移酶介导的dUTP-生物素末端标记来评估光感受器细胞的存活和凋亡。
在实验性视网膜脱离后第1天检测到光感受器细胞凋亡，凋亡细胞数量在第3天达到峰值，并在第7天下降。当在视网膜脱离前24小时甚至脱离后24小时给予米诺环素治疗时，可显著减少与视网膜脱离相关的凋亡光感受器细胞数量。
我们的数据表明，米诺环素可能对治疗与视网膜脱离相关的光感受器变性有用，即使在视网膜脱离后24小时给药。
黄斑脱离性视网膜脱离患者使用米诺环素可能预防光感受器凋亡和胶质细胞增殖，改善最终视觉结果。
