Institute of Animal Nutrition and Physiology, Christian-Albrechts-University of Kiel, Hermann-Rodewald-Strasse 9, 24098 Kiel, Germany.
J Clin Biochem Nutr. 2009 Nov;45(3):315-21. doi: 10.3164/jcbn.08-248. Epub 2009 Oct 28.
The standardised Ginkgo biloba extract EGb761 is known for its potential beneficial effects in the prevention and therapy of neurodegenerative disorders including Alzheimer's disease (AD). However, the molecular mechanisms and the specific role of its constituents are largely unknown. The aim of the present feeding trial was to investigate the effects of EGb761 and its major constituents on the expression of genes encoding for proteins involved in the pathogenesis of AD in mouse brain. Six month old C57B6 mice were fed semi synthetic diets enriched with either EGb761 or one of its main fractions, flavonols and terpenelactones, respectively, over a period of 4 weeks. Thereafter, mRNA of alpha-secretase, neprilysin, amyloid precursor protein (App), App binding protein-1 and acetylcholine esterase was quantified in hippocampus and cortex. EGb761 and its flavonol fraction had no effects on relative mRNA levels of the respective genes in mouse brain. However, the terpenelactone fraction significantly decreased the mRNA levels of App in the hippocampus. Taken together, a 4 week dietary treatment with EGb761 or its main fractions had only moderate effects on mRNA levels of AD related genes in cortex and hippocampus of mice.
标准的银杏叶提取物 EGb761 以其在预防和治疗神经退行性疾病（包括阿尔茨海默病（AD））方面的潜在有益作用而闻名。然而，其成分的分子机制和特定作用在很大程度上尚不清楚。本喂养试验的目的是研究 EGb761 及其主要成分对参与 AD 发病机制的蛋白质编码基因在小鼠大脑中的表达的影响。6 月龄 C57B6 小鼠用富含 EGb761 或其主要成分之一（分别为类黄酮和萜烯内酯）的半合成饮食喂养 4 周。此后，在海马体和皮质中定量测定α-分泌酶、内肽酶、淀粉样前体蛋白（App）、App 结合蛋白-1 和乙酰胆碱酯酶的 mRNA。EGb761 及其类黄酮部分对小鼠脑中相应基因的相对 mRNA 水平没有影响。然而，萜烯内酯部分显著降低了海马体中 App 的 mRNA 水平。总之，用 EGb761 或其主要成分进行 4 周的饮食处理仅对小鼠大脑皮质和海马体中 AD 相关基因的 mRNA 水平有适度影响。
