Department of Instrumentation and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Anal Bioanal Chem. 2010 Jan;396(2):943-8. doi: 10.1007/s00216-009-3246-2. Epub 2009 Nov 10.
A novel small-volume fiber-optic evanescent-wave absorption sensor based on the Griess-Ilosvay reaction has been developed and evaluated for nitrite determination. The sensor was constructed by inserting a decladded optical fiber into a transparent capillary to form an annular column microchannel. The Evanescent wave (EW) field produced on the optical fiber core surface penetrated into the surrounding medium and interacted with the azo dye, which was generated by the reaction of nitrite and nitrite-sensitive reagents. The detector was designed to be parallel to the axis of the optical fiber. The defined absorbance was linear with the concentration of nitrite in the range from 0.05 to 10 mg L(-1), and the detection limit was 0.02 mg L(-1) (3sigma) with the relative standard deviation (RSD) of 2.6% (n = 8). The present sensor was successfully used to determine nitrite in real samples of mineral water, tap water, rain water, and seawater. The results were consistent with the data obtained by standard spectrophotometric method, showing potential of the proposed sensor for practical application.
一种基于格里斯-伊洛斯基反应的新型小体积光纤倏逝波吸收传感器已被开发出来，并用于亚硝酸盐的测定。该传感器通过将去包层光纤插入透明毛细管中形成环形柱微通道来构建。光纤芯表面产生的倏逝波（EW）场穿透到周围介质中，并与由亚硝酸根和亚硝酸根敏感试剂反应生成的偶氮染料相互作用。该探测器设计为与光纤的轴平行。在 0.05 至 10mg/L 的范围内，定义的吸光度与亚硝酸盐浓度呈线性关系，检测限为 0.02mg/L（3σ），相对标准偏差（RSD）为 2.6%（n=8）。该传感器已成功用于测定矿泉水、自来水、雨水和海水中的亚硝酸盐。结果与标准分光光度法获得的数据一致，表明该传感器具有实际应用的潜力。
