Xiong Yan, Wang Cheng-Jie, Tao Tao, Duan Ming, Fang Shen-Wen, Zheng Min
State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation, Southwest Petroleum University, Chengdu, Sichuan, 610500, China.
Oil and Gas Field Applied Chemistry Key Laboratory of Sichuan Province, Southwest Petroleum University, Chengdu, Sichuan, 610500, China.
Anal Bioanal Chem. 2016 May;408(13):3413-23. doi: 10.1007/s00216-016-9415-1. Epub 2016 Mar 3.
A microfluidic-capillary-waveguide-coupled fiber-optic sensor was developed for colorimetric determination of hazardous nitrite based on the Griess-Ilosvay reaction. The sensor was modularly designed by use of a light-emitting diode as the light source, silica fiber as the light transmission element, and a capillary waveguide tube as the light reaction flow cell. With the light interacting with the azo dye generated by the Griess-Ilosvay reaction between nitrite and Griess reagents, nitrite could be determined by a colorimetric method according to Beer's law. By use of the inexpensive and micro-sized elements mentioned above, the sensor provided a new low-cost and portable method for in situ and online measurement of nitrite. The sensor had a wide linear range for nitrite from 0.02 to 1.8 mg L(-1) and a low detection limit of 7 μg L(-1) (3σ), with a relative standard deviation of 0.37% (n = 10). With a low reagent demand of 200 μL, a short response time of 6.24 s, and excellent selectivity, the sensor is environmentally friendly and has been applied to nitrite determination in different water samples. The results were compared with those obtained by conventional spectrophotometry and ion chromatography, indicating the sensor's potential for practical applications.
基于格里斯-伊洛夫西反应，开发了一种微流控-毛细管波导耦合光纤传感器用于比色法测定有害亚硝酸盐。该传感器采用模块化设计，以发光二极管作为光源，石英光纤作为光传输元件，毛细管波导管作为光反应流通池。通过光与亚硝酸盐和格里斯试剂之间的格里斯-伊洛夫西反应生成的偶氮染料相互作用，根据比尔定律，可采用比色法测定亚硝酸盐。利用上述廉价且微型化的元件，该传感器提供了一种用于原位和在线测量亚硝酸盐的新型低成本便携式方法。该传感器对亚硝酸盐的线性范围宽，为0.02至1.8 mg L(-1)，检测限低至7 μg L(-1)（3σ），相对标准偏差为0.37%（n = 10）。该传感器试剂需求量低至200 μL，响应时间短至6.24 s，选择性优异，对环境友好，已应用于不同水样中亚硝酸盐的测定。将结果与传统分光光度法和离子色谱法获得的结果进行比较，表明该传感器具有实际应用潜力。
