Lemaire L, Heinlein U A
Institut für Genetik, Heinrich-Heine-Universität, Düsseldorf, Fed. Rep. Germany.
Life Sci. 1991;48(4):365-72. doi: 10.1016/0024-3205(91)90557-r.
Temperature-induced effects on the synthesis of murine testicular proteins were investigated by one- and two-dimensional SDS polyacrylamide gel electrophoresis. Newly synthesized proteins were monitored by incorporation of 35S-methionine and autoradiography. Three heat shock responsive proteins, which are differently affected by elevated temperatures, are described. These proteins represent special examples for how testicular cells respond to environmental stress. One of these proteins, HSl36, is synthesized and secreted at 38 degrees C, whereas at lower, scrotal temperatures it is not detectable. HSlD74 protein is synthesized at elevated temperatures, but only in prepuberal testis, not in adult. Synthesis of the third example, HSR28, is decreased within the seminiferous tubules, but only in those regions which bear cell associations of the elongation stage. These results indicate that the use of DNA probes of the 'heat shock'-gene family might not be sufficient to describe the molecular reasons for impaired spermatogenesis following hyperthermia.
通过一维和二维SDS聚丙烯酰胺凝胶电泳研究了温度对小鼠睾丸蛋白质合成的影响。通过掺入35S-甲硫氨酸和放射自显影监测新合成的蛋白质。描述了三种对温度升高有不同影响的热休克反应蛋白。这些蛋白质代表了睾丸细胞如何应对环境压力的特殊例子。其中一种蛋白质HS136在38摄氏度时合成并分泌，而在较低的阴囊温度下则无法检测到。HS1D74蛋白在温度升高时合成，但仅在青春期前睾丸中合成，成年睾丸中不合成。第三个例子HSR28的合成在生精小管内减少，但仅在那些处于伸长阶段细胞关联的区域。这些结果表明，使用“热休克”基因家族的DNA探针可能不足以描述高温后精子发生受损的分子原因。
