Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Hayashi-cho 2217-14, Takamatsu 761-0395, Japan.
Anal Chem. 2009 Dec 15;81(24):10055-60. doi: 10.1021/ac902014c.
We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from alpha1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.
我们开发了一种使用聚甲基丙烯酸甲酯 (PMMA) 通道静态涂覆疏水改性羟乙基纤维素 (HM-HEC) 进行糖蛋白相互作用快速筛选的新方法。我们发现，水稳定的 PMMA 表面上的 HM-HEC 自组装单层 (SAM) 允许在没有 HM-HEC 的情况下使用 20mM 磷酸盐快速且可重复地分离糖样。讨论了 PMMA 表面上动态和静态涂层的基础机制。成功地在 PMMA 通道的静态 HM-HEC SAM 上实现了同时分析来自α1-酸性糖蛋白的复杂碳水化合物混合物与蛋白质之间的分子相互作用。
