Center for Asian Traditional Medicine, Tohoku University Graduate School of Medicine, Aoba, Sendai 980-8574, Japan.
Gynecol Endocrinol. 2009 Jun;25(6):403-9. doi: 10.1080/09513590902730804.
Uterine leiomyomas are the most common gynecological benign tumor and greatly affect reproductive health and well-being. They are the predominant indication for hysterectomy in premenopausal women. Current epidemiological study reported that soy products intake is inversely associated with diseases leading to hysterectomy. Genistein is a soy-derived phytoestrogen and its inhibitory effect on leiomyoma cell proliferation is reported. In this study, we investigated the siginificant inhibitory effect of genistein on estradiol (E(2))-induced leiomyoma cells proliferation.
The Eker rat-derived uterine leiomyoma cell line ELT-3 cells were used. Cell proliferation was assessed by counting the number of cells. The expression of estrogen receptors and peroxisome proliferator-activated receptor-gamma (PPARgamma) was evaluated by Western blot analysis.
PPARgamma was expressed in ELT-3 cells and genistein acted as PPARgamma ligand. This inhibitory effect of genistein was attenuated by the treatment of cells with PPARgamma antagonist bisphenol A diglycidyl ether (BADGE) or GW9662.
These experimental findings in vitro show that the repressive effect of genistein on E(2)-induced ELT-3 cell proliferation is through the activation of PPARgamma. Genistein may be useful as an alternative therapy for leiomyoma.
子宫肌瘤是最常见的妇科良性肿瘤，严重影响生殖健康和生活质量。它是绝经前妇女行子宫切除术的主要指征。目前的流行病学研究报告称，大豆制品的摄入与导致子宫切除术的疾病呈负相关。染料木黄酮是一种大豆来源的植物雌激素，其对子宫肌瘤细胞增殖的抑制作用已有报道。在这项研究中，我们研究了染料木黄酮对雌二醇（E（2））诱导的子宫肌瘤细胞增殖的显著抑制作用。
使用 Eker 大鼠来源的子宫肌瘤细胞系 ELT-3 细胞。通过计数细胞数量来评估细胞增殖。通过 Western blot 分析评估雌激素受体和过氧化物酶体增殖物激活受体-γ（PPARγ）的表达。
PPARγ在 ELT-3 细胞中表达，染料木黄酮作为 PPARγ配体。用 PPARγ拮抗剂双酚 A 二缩水甘油醚（BADGE）或 GW9662 处理细胞可减弱染料木黄酮的这种抑制作用。
这些体外实验结果表明，染料木黄酮对 E（2）诱导的 ELT-3 细胞增殖的抑制作用是通过激活 PPARγ 实现的。染料木黄酮可能可作为子宫肌瘤的一种替代疗法。
