Everett Anthany L, Scholthof Herman B, Scholthof Karen-Beth G
Department of Plant Pathology and Microbiology, 2132 TAMU, Texas A&M University, College Station, TX 77843, USA.
Virology. 2010 Jan 5;396(1):37-46. doi: 10.1016/j.virol.2009.10.006. Epub 2009 Nov 10.
The coat protein of satellite panicum mosaic virus (SPCP) is known to effectively protect its cognate RNA from deleterious events, and here, we tested its stabilizing potential for heterologous virus-based gene vectors in planta. In support of this, a Potato virus X (PVX) vector carrying the SPMV capsid protein (PVX-SPCP) gene was stable for at least three serial systemic passages through Nicotiana benthamiana. To test the effect of SPCP in trans, PVX-SPCP was co-inoculated onto N. benthamiana together with a Tomato bushy stunt virus (TBSV) vector carrying a green fluorescent protein (GFP) gene that normally does not support systemic GFP expression. In contrast, co-inoculation of TBSV-GFP plus PVX-SPCP resulted in GFP accumulation and concomitant green fluorescent spots in upper, non-inoculated leaves in a temperature-responsive manner. These results suggest that the multifaceted SPMV CP has intriguing effects on virus-host interactions that surface in heterologous systems.
已知卫星黍花叶病毒（SPCP）的外壳蛋白能有效保护其同源RNA免受有害事件影响，在此，我们测试了其在植物中对基于异源病毒的基因载体的稳定潜力。为此，携带SPMV衣壳蛋白（PVX-SPCP）基因的马铃薯X病毒（PVX）载体在通过本氏烟草进行至少三轮连续系统传代后仍保持稳定。为了测试SPCP的反式作用效果，将PVX-SPCP与携带绿色荧光蛋白（GFP）基因的番茄丛矮病毒（TBSV）载体共同接种到本氏烟草上，该TBSV载体通常不支持GFP的系统表达。相反，TBSV-GFP与PVX-SPCP共同接种导致GFP积累，并在未接种的上部叶片中以温度响应方式出现绿色荧光斑点。这些结果表明，具有多方面作用的SPMV CP对异源系统中表面的病毒-宿主相互作用具有有趣的影响。
