Tehran University of Medical Sciences, Tehran 14155, Iran.
Diagn Microbiol Infect Dis. 2010 Mar;66(3):285-91. doi: 10.1016/j.diagmicrobio.2009.10.006. Epub 2009 Nov 10.
Surface gene mutants of hepatitis B virus (HBV) have been reported in a variety of patient groups. Because of limited data regarding these mutations in patients with occult HBV infections; we aimed to determine these mutations among high-risk patients with occult HBV infection. The presence of HBV-DNA was determined in patients with isolated anti-HBc by real-time polymerase chain reaction (PCR). Then, surface gene region was amplified by nested PCR and mutations were analyzed after sequencing. The mutations that resulted in nonfunctional hepatitis B surface antigen (HBsAg) were insertion of single nucleotide in 2 cases, which causes frameshift and single-nucleotide replacement, and premature stop codons at Leu15 and Gly10 in the other 2 cases. Amino acid substitution at amino acid position 207(S207N) was found in the other isolates. Our study suggested that "a" region mutations did not play a major role in HBsAg detection, and other genetic and nongenetic factors may be responsible for failure to detect HBsAg by routine laboratory tests.
已在各种患者群体中报道了乙型肝炎病毒 (HBV) 的表面基因突变。由于隐匿性 HBV 感染患者的这些突变数据有限，我们旨在确定隐匿性 HBV 感染高危患者中的这些突变。采用实时聚合酶链反应 (PCR) 法检测抗-HBc 单独阳性患者的 HBV-DNA。然后，通过巢式 PCR 扩增表面基因区域，并在测序后分析突变。导致乙型肝炎表面抗原 (HBsAg) 无功能的突变有 2 例为单个核苷酸插入，导致移码和单个核苷酸替换，另外 2 例为亮氨酸 15 位和甘氨酸 10 位的提前终止密码子。在其他分离物中发现了氨基酸位置 207(S207N)的氨基酸取代。本研究表明，“a”区突变在 HBsAg 检测中不起主要作用，其他遗传和非遗传因素可能导致常规实验室检测未能检测到 HBsAg。
