Otte A P, Kramer I M, Durston A J
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Science. 1991 Feb 1;251(4993):570-3. doi: 10.1126/science.1990433.
The limited competence of embryonic tissue to respond to an inductive signal has an essential, regulatory function in embryonic induction. The molecular basis for the competence of Xenopus ectoderm to differentiate into neural tissue was investigated. Dorsal mesoderm or 12-O-tetradecanoyl phorbol-13-acetate (TPA) caused in vivo activation of protein kinase C (PKC) and neural differentiation mainly in dorsal ectoderm and to a lesser extent in ventral ectoderm. These data correlate with the observations that PKC preparations from dorsal and ventral ectoderm differ, the dorsal PKC preparation being more susceptible to activation by TPA and diolein than is the ventral PKC preparation. Monoclonal antibodies against the bovine PKC alpha plus beta or gamma isozymes immunostained dorsal and ventral ectoderm, respectively, which suggests different localizations of PKC isozymes. These results suggest that PKC participates in the establishment of embryonic competence.
胚胎组织对诱导信号作出反应的能力有限，这在胚胎诱导过程中具有重要的调节功能。研究了非洲爪蟾外胚层分化为神经组织的能力的分子基础。背侧中胚层或12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）在体内激活蛋白激酶C（PKC），并主要在背侧外胚层诱导神经分化，在腹侧外胚层诱导程度较小。这些数据与以下观察结果相关：背侧和腹侧外胚层的PKC制剂不同，背侧PKC制剂比腹侧PKC制剂更容易被TPA和二油精激活。针对牛PKCα加β或γ同工酶的单克隆抗体分别对背侧和腹侧外胚层进行免疫染色，这表明PKC同工酶的定位不同。这些结果表明PKC参与了胚胎感受态的建立。
