基于 DNA 的电化学生物传感器用于无标记监测蛋白激酶的活性和抑制作用。
A DNA-based electrochemical strategy for label-free monitoring the activity and inhibition of protein kinase.
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
出版信息
Chem Commun (Camb). 2009 Dec 7(45):6946-8. doi: 10.1039/b913943e. Epub 2009 Oct 16.
DOI:10.1039/b913943e
PMID:19904356
Abstract
A novel label-free electrochemical strategy for monitoring the activity and inhibition of protein kinase is developed, based on the linkage between the phosphorylated peptide and DNA functionalized Au nanoparticles (DNA-AuNPs) by Zr(4+) and the chronocoulometric response of Ru(NH(3))(6) absorbed on the DNA-AuNPs.
摘要
一种新型的无标记电化学策略被开发用于监测蛋白激酶的活性和抑制，该策略基于磷酸化肽与通过 Zr(4+) 连接的 DNA 功能化金纳米粒子（DNA-AuNPs），以及吸附在 DNA-AuNPs 上的 Ru(NH(3))(6) 的计时库仑响应。
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