水溶性聚合物保护的脂质体 2000/DNA 复合物用于固相转染。

Water soluble polymer protected lipofectamine 2000/DNA complexes for solid-phase transfection.

机构信息

Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, PR China.

出版信息

Macromol Biosci. 2009 Dec 8;9(12):1262-71. doi: 10.1002/mabi.200900255.

DOI:10.1002/mabi.200900255

PMID:19904725

Abstract

A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a water-soluble polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in polymer films. The degradation of the polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.

摘要

已使用快速降解的胆酸功能化星型聚（DL-丙交酯）来制备聚合物膜，以支持介导固相转染的 Lipofectamine 2000/DNA 复合物。为了提高基因表达活性，加入了水溶性聚合物聚-α，β-[N-（2-羟乙基）-L-天冬酰胺（PHEA）以保护复合物。在 293T 细胞、HeLa 细胞和 3T3 细胞中的体外基因转染表明，包裹在聚合物膜中的沉积的 Lipofectamine 2000/DNA 复合物可以有效地介导基因转染。在基因转染过程中聚合物膜的降解并没有对基因表达产生任何不利影响。