Tomofuji Takaaki, Ekuni Daisuke, Irie Koichiro, Azuma Tetsuji, Endo Yasumasa, Tamaki Naofumi, Sanbe Toshihiro, Murakami Jun, Yamamoto Tatsuo, Morita Manabu
Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
J Periodontol. 2009 Nov;80(11):1799-808. doi: 10.1902/jop.2009.090270.
Oxidative stress affects the progression of periodontitis. Cocoa is a rich source of flavonoids with antioxidant properties, which could suppress gingival oxidative stress in periodontal lesions. The purpose of the present study was to investigate the effects of a cocoa-enriched diet on gingival oxidative stress in a rat-periodontitis model.
In this 4-week study, rats were divided into three groups (n = 8/group): a control group (fed a regular diet) and two periodontitis groups (fed a regular diet or cocoa-enriched diet [10% of food intake]). Periodontitis was induced by ligature placement around the mandibular first molars. Serum levels for reactive oxygen metabolites were measured at baseline and 2 and 4 weeks. At 4 weeks, the levels of 8-hydroxydeoxyguanosine and reduced/oxidized glutathione ratio were determined to evaluate gingival oxidative damage and antioxidant status, respectively.
Rats with experimental periodontitis that were fed a regular diet showed an increase in the level of serum reactive oxygen metabolites in a time-dependent manner. These rats also had an increased 8-hydroxydeoxyguanosine level and decreased reduced/oxidized glutathione ratio in the gingival tissue, inducing alveolar bone loss and polymorphonuclear leukocyte infiltration. Although experimental periodontitis was induced in the rats fed a cocoa-enriched diet, they did not show impairments in serum reactive oxygen metabolite level and gingival levels for 8-hydroxydeoxyguanosine and reduced/oxidized glutathione ratio. Alveolar bone loss and polymorphonuclear leukocyte infiltration after ligature placement were also inhibited by cocoa intake.
Consuming a cocoa-enriched diet could diminish periodontitis-induced oxidative stress, which, in turn, might suppress the progression of periodontitis.
氧化应激影响牙周炎的进展。可可富含具有抗氧化特性的类黄酮，可抑制牙周病变中的牙龈氧化应激。本研究的目的是探讨富含可可的饮食对大鼠牙周炎模型中牙龈氧化应激的影响。
在这项为期4周的研究中，大鼠被分为三组（每组n = 8）：对照组（喂食常规饮食）和两个牙周炎组（分别喂食常规饮食或富含可可的饮食[食物摄入量的10%]）。通过在下颌第一磨牙周围结扎诱导牙周炎。在基线以及第2周和第4周测量血清活性氧代谢产物水平。在第4周时，分别测定8-羟基脱氧鸟苷水平和还原型/氧化型谷胱甘肽比率，以评估牙龈氧化损伤和抗氧化状态。
喂食常规饮食的实验性牙周炎大鼠血清活性氧代谢产物水平呈时间依赖性升高。这些大鼠牙龈组织中的8-羟基脱氧鸟苷水平也升高，还原型/氧化型谷胱甘肽比率降低，导致牙槽骨丧失和多形核白细胞浸润。虽然喂食富含可可饮食的大鼠也诱导了实验性牙周炎，但它们的血清活性氧代谢产物水平以及牙龈组织中的8-羟基脱氧鸟苷水平和还原型/氧化型谷胱甘肽比率均未出现受损情况。结扎后牙槽骨丧失和多形核白细胞浸润也受到可可摄入的抑制。
食用富含可可的饮食可减轻牙周炎诱导的氧化应激，进而可能抑制牙周炎的进展。
