Obstetrics & Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai 200011, China.
Placenta. 2010 Jan;31(1):60-6. doi: 10.1016/j.placenta.2009.10.002. Epub 2009 Nov 11.
This study aimed to investigate the location and function of tau isoform of 14-3-3 proteins in human trophoblast. 14-3-3 tau was localized in human cytotrophoblast cells, but not in syncytiotrophoblast cells in both first trimester and term placenta by immunochemistry stain. Forskolin-induced cell fusion (BeWo cells) confirmed that 14-3-3 tau was decreased during trophoblast differentiation. Forskolin-induced differentiation was stimulated by small-interfering (si) RNA induced down-regulation of 14-3-3 tau, contrarily, it was suppressed by plasmid induced upregulation of 14-3-3 tau in BeWo cells. When BeWo cells were treated with 14-3-3 tau siRNA, an increase in protein concentration of cell cycle inhibitor p27kip1 and a decrease in protein concentration of proliferating cell nuclear antigen, as well as activation of the extracellular signal-regulated kinase (ERK) pathway, were also noticed. These findings suggest that 14-3-3 tau might be mediated trophoblast differentiation through cell cycle regulation.
本研究旨在探讨 14-3-3 蛋白 tau 异构体在人滋养层中的位置和功能。免疫组织化学染色显示，14-3-3 tau 定位于人细胞滋养层细胞中，但不在早孕期和足月胎盘的合体滋养层细胞中。佛司可林诱导的细胞融合（BeWo 细胞）证实，14-3-3 tau 在滋养层分化过程中减少。佛司可林诱导的分化受到 14-3-3 tau 的小干扰 RNA 下调的刺激，相反，它受到质粒诱导的 14-3-3 tau 上调的抑制在 BeWo 细胞中。当 BeWo 细胞用 14-3-3 tau siRNA 处理时，细胞周期抑制剂 p27kip1 的蛋白浓度增加，增殖细胞核抗原的蛋白浓度降低，细胞外信号调节激酶 (ERK) 途径被激活。这些发现表明，14-3-3 tau 可能通过细胞周期调节介导滋养层分化。
