通过转移缓慢分裂的人类造血干细胞来改善 T 和 B 细胞的恢复。

Improved T and B cell recovery by the transfer of slowly dividing human hematopoietic stem cells.

机构信息

Department of Tumor Cell Biology, University Hospital of Surgery and German Cancer Research Centre, University of Heidelberg, Heidelberg, Germany.

出版信息

Leuk Res. 2010 May;34(5):622-30. doi: 10.1016/j.leukres.2009.10.015. Epub 2009 Nov 10.

DOI:10.1016/j.leukres.2009.10.015

PMID:19906424

Abstract

Human hematopoietic stem cells giving rise to long term initiating cells in vitro are enriched in a CD34(+) slow dividing fraction (SDF). Here, we tested reconstitution and multilineage differentiation of this CD34(+) SDF in NOD/SCID mice. In the bone marrow a slightly higher percentage of human hematopoietic progenitors were recovered after the transfer of the SDF compared to the fast dividing fraction. Instead, T cell maturation in the rudimentary thymus and lymph node repopulation was only initiated by the SDF. The capacity of the SDF to differentiate and mature in the patients' thymus could provide an advantage in immunocompetence recovery.

摘要

人类造血干细胞在体外产生长期起始细胞的过程中，富集在 CD34(+) 慢分裂分数（SDF）中。在这里，我们测试了该 CD34(+) SDF 在 NOD/SCID 小鼠中的重建和多谱系分化。在骨髓中，与快速分裂分数相比，转移 SDF 后可回收稍高比例的人类造血祖细胞。相反，只有 SDF 才能启动原始胸腺和淋巴结中的 T 细胞成熟和再定植。SDF 在患者胸腺中分化和成熟的能力可能为免疫功能恢复提供优势。