UPMC University Paris 06, UMR 7621, Laboratoire ARAGO, CNRS, UMR 7621, Avenue Fontaulé, BP44, F-66650 Banyuls-sur-Mer, France.
Environ Pollut. 2010 Mar;158(3):663-73. doi: 10.1016/j.envpol.2009.10.026. Epub 2009 Nov 10.
The dynamics of total and active microbial communities were studied in seawater microcosms amended with crude or diesel oil at different temperatures (25, 10 and 4 degrees C) in the presence/absence of organic fertilization (Inipol EAP 22). Total and hydrocarbon-degrading microbes were enumerated by fluorescence microscopy and Most Probable Number (MPN) method, respectively. Total (16S rDNA-based) vs. active (16S rRNA) bacterial community structure was monitored by Capillary-Electrophoresis Single Strand Conformation Polymorphism (CE-SSCP) fingerprinting. Hydrocarbons were analyzed after 12 weeks of incubation by gas chromatography-mass spectrometry. Total and hydrocarbon-degrading microbial counts were highly influenced by fertilization while no important differences were observed between temperatures. Higher biodegradation levels were observed in fertilized microcosms. Temperature and fertilization induced changes in structure of total bacterial communities. However, fertilization showed a more important effect on active bacterial structure. The calculation of Simpson's diversity index showed similar trends among temperatures whereas fertilization reduced diversity index of both total and active bacterial communities.
研究了在 25、10 和 4°C 不同温度下，添加原油或柴油的海水微宇宙中总微生物和活性微生物群落的动态变化，以及是否存在有机施肥（Inipol EAP 22）。通过荧光显微镜和最可能数（MPN）方法分别对总微生物和烃类降解微生物进行计数。通过毛细管电泳单链构象多态性（CE-SSCP）指纹图谱监测总（基于 16S rDNA）与活性（16S rRNA）细菌群落结构。在孵育 12 周后，通过气相色谱-质谱法分析烃类。总微生物和烃类降解微生物的数量受到施肥的高度影响，而温度之间没有观察到重要差异。在施肥微宇宙中观察到更高的生物降解水平。温度和施肥引起总细菌群落结构的变化。然而，施肥对活性细菌结构的影响更为重要。辛普森多样性指数的计算表明，在不同温度下表现出相似的趋势，而施肥降低了总细菌和活性细菌群落的多样性指数。
