Wong Raymond C B, Pébay Alice
Department of Biological Chemistry, University of California Irvine, Irvine, CA, USA.
Methods Mol Biol. 2010;584:211-28. doi: 10.1007/978-1-60761-369-5_12.
Gap junctional intercellular communication (GJIC) has been described in different cell types including stem cells and has been involved in different biological events. GJIC is required for mouse embryonic stem cell maintenance and proliferation, and various studies suggest that functional GJIC is a common characteristic of human embryonic stem cells (hESC) maintained in different culture conditions. This chapter introduces methods to study gap junctions in hESC, from expression of gap junction proteins to functional study of GJIC in hESC proliferation, apoptosis, colony growth, and pluripotency.
缝隙连接细胞间通讯（GJIC）已在包括干细胞在内的不同细胞类型中被描述，并参与了不同的生物学事件。小鼠胚胎干细胞的维持和增殖需要GJIC，并且各种研究表明功能性GJIC是在不同培养条件下维持的人类胚胎干细胞（hESC）的共同特征。本章介绍了研究hESC中缝隙连接的方法，从缝隙连接蛋白的表达到GJIC在hESC增殖、凋亡、集落生长和多能性方面的功能研究。
