长时间机械加载后组织工程表皮替代物中的细胞因子释放

Cytokine release in tissue-engineered epidermal equivalents after prolonged mechanical loading.

作者信息

Cornelissen Lisette H, Bronneberg Debbie, Gibbs Susan, Bouten Carlijn V C, Oomens Cees W J

机构信息

Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.

出版信息

Methods Mol Biol. 2010;585:335-44. doi: 10.1007/978-1-60761-380-0_23.

DOI:10.1007/978-1-60761-380-0_23

PMID:19908014

Abstract

Prolonged mechanical loading of soft tissues may result in degeneration of these tissues, resulting in formation of pressure ulcers. The risk assessment of individuals might be improved by including measurements of the tissue response to mechanical loading. Cytokines, which are released by the top layer of the skin upon chemical irritation, might be used to determine the epidermal response to mechanical loading. This chapter describes methods to measure the release of cytokines IL-1 alpha, IL-1RA, and IL-8 from tissue-engineered epidermal equivalents in response to sustained mechanical loading. A custom-built loading device was used to apply load to the epidermal equivalents and the cytokines were measured using an enzyme-linked immunosorbent assay.

摘要

软组织的长期机械负荷可能导致这些组织退化，进而形成压疮。通过纳入对组织对机械负荷反应的测量，可能会改善个体的风险评估。细胞因子是皮肤表层在化学刺激下释放的物质，可用于确定表皮对机械负荷的反应。本章描述了测量组织工程表皮替代物在持续机械负荷下白细胞介素-1α（IL-1α）、白细胞介素-1受体拮抗剂（IL-1RA）和白细胞介素-8（IL-8）细胞因子释放的方法。使用定制的加载装置对表皮替代物施加负荷，并使用酶联免疫吸附测定法测量细胞因子。