Centro de Química, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Amino Acids. 2010 Jun;39(1):121-33. doi: 10.1007/s00726-009-0383-z. Epub 2009 Nov 12.
Aiming at the development of new benzopyran-based photocleavable protecting groups, novel chloromethylated and hydroxymethylated 2-oxo-2H-benzo[h]benzopyran derivatives bearing a methoxy substituent were designed and used in the synthesis of a series of fluorescent bioconjugates, by linking through an ester or urethane bond to several model neurotransmitter amino acids (glycine, alanine, beta-alanine and gamma-aminobutyric acid, GABA). The resulting fluorescent bioconjugates with emission in the visible range and high fluorescent quantum yields, were subjected to photocleavage reaction in methanol/HEPES buffer (80:20) solution at different wavelengths of irradiation (250, 300, 350 and 419 nm) and photocleavage kinetic data were obtained.
针对新型苯并吡喃类光解保护基团的发展，设计了新型氯甲基和羟甲基化的 2-氧代-2H-苯并[h]苯并吡喃衍生物，带有甲氧基取代基，并用于通过酯或氨酯键与几种模型神经递质氨基酸（甘氨酸、丙氨酸、β-丙氨酸和γ-氨基丁酸（GABA））合成一系列荧光生物缀合物。得到的在可见范围内发射且具有高荧光量子产率的荧光生物缀合物，在甲醇/HEPES 缓冲液（80:20）溶液中在不同的辐照波长（250、300、350 和 419nm）下进行光解反应，并获得光解动力学数据。
