Department of Neurology, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
J Neurosci Res. 2010 Apr;88(5):945-56. doi: 10.1002/jnr.22263.
The distribution of neural precursor cells (NPCs) in adult mice brain has so far not been described. Therefore, we investigated the distribution of NPCs by analyzing the nestin-containing cells (NCCs) in distinct brain regions of adult nestin second-intron enhancer-controlled LacZ reporter transgenic mice through LacZ staining. Results showed that NCCs existed in various regions of adult mouse brain. In cerebellum, the greatest number of NCCs existed in cortex of the simple lobule, followed by cortex of the cerebellar lobule. In olfactory bulb, NCCs were most numerous in the granular cell layer, followed by the mitral cell layer and the internal plexiform, glomerular, and external plexiform layers. In brain nuclei (nu), NCCs were most numerous in the marginal nu, followed by the brainstem and diencephalon nu. NCCs in sensory nu of brainstem were more numerous than in motor nu, and NCCs in the dorsal of sensory nu were more numerous than in the ventral part. In brain ventricle systems, NCCs were largely distributed in the center of and external to the lateral ventricle, the inferior part of the third ventricle, the dorsal and inferior parts of the fourth ventricle, and the gray matter around the cerebral aqueduct. NCCs in the left vs. right brain were not significantly different. These data collectively indicate that NCCs were extensively distributed in the cerebellum and olfactory bulb, the partial nu of the marginal system, the partial brain nu adjacent to brain ventricle systems, the subependymal zone, and the cerebral cortex around the marginal lobe and were a potential source of NPCs.
神经前体细胞（NPCs）在成年小鼠大脑中的分布目前尚未描述。因此，我们通过对 nestin 第二内含子增强子控制的 LacZ 报告转基因小鼠不同脑区的 nestin 阳性细胞（NCCs）进行 LacZ 染色，分析 NPCs 的分布。结果显示，NCCs 存在于成年小鼠大脑的不同区域。在小脑，NCCs 数量最多的区域是简单小叶皮质，其次是小脑小叶皮质。在嗅球，NCCs 数量最多的区域是颗粒细胞层，其次是僧帽细胞层和内丛状层、肾小球层和外丛状层。在脑核（nu）中，NCCs 数量最多的区域是边缘核，其次是脑干和间脑核。脑干感觉核中的 NCCs 比运动核中的多，感觉核背侧的 NCCs 比腹侧的多。在脑室系统中，NCCs 主要分布在侧脑室中心和外部、第三脑室下部、第四脑室背侧和下部以及脑导水管周围的灰质。左右大脑中的 NCCs 没有显著差异。这些数据表明，NCCs 广泛分布于小脑和嗅球、边缘系统的部分核、邻近脑室系统的部分脑核、室管膜下区以及边缘叶周围的大脑皮质，是 NPCs 的潜在来源。
