Pei Xiaoping, Zhao Yuanjin, Zhao Xiangwei, Xu Hua, Chen Baoan, Gu Zhongze
School of Clinical Medicine, Department of Hematology, Affiliated Zhongda Hospital, Southeast University, Nanjing 210009, China.
J Nanosci Nanotechnol. 2009 Nov;9(11):6320-5. doi: 10.1166/jnn.2009.1335.
The paper presented a novel multiplex chemiluminescence (CL) immunoassay method, which was based on silica colloidal crystals beads (SCCBs) as carriers using enhanced chemiluminescence (CL) detection of a horseradish peroxidase (HRP) catalyzing the luminal reaction. The monodisperse and size-controlled SCCBs were fabricated by a microfluidic device. The experimental conditions were optimized and analytical performance was studied. Results showed that the SCCBs as supports were much sensitive (0.05 ng/ml IgG) than the glass beads (18 ng/ml IgG) and the planar carriers (125 ng/ml IgG). A multiplex immunoassay suggested the feasibility of high throughput applications. This novel immunoassay system provided a simple, sensitive and low-cost approach for multianalyte immunoassay without using of expensive array detector.
该论文提出了一种新型的多重化学发光（CL）免疫分析方法，该方法基于以二氧化硅胶体晶体微球（SCCBs）为载体，利用辣根过氧化物酶（HRP）催化鲁米诺反应的增强化学发光（CL）检测。通过微流控装置制备了单分散且尺寸可控的SCCBs。对实验条件进行了优化并研究了分析性能。结果表明，作为载体的SCCBs比玻璃微球（18 ng/ml IgG）和平板载体（125 ng/ml IgG）更灵敏（0.05 ng/ml IgG）。多重免疫分析表明了高通量应用的可行性。这种新型免疫分析系统为多分析物免疫分析提供了一种简单、灵敏且低成本的方法，无需使用昂贵的阵列检测器。
