Zhao Yuanjin, Zhao Xiangwei, Sun Cheng, Li Juan, Zhu Rong, Gu Zhongze
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Anal Chem. 2008 Mar 1;80(5):1598-605. doi: 10.1021/ac702249a. Epub 2008 Feb 5.
We developed a new kind of suspension array for multiplexed immunoassays using silica colloidal crystal beads (SCCBs) as coding carriers. The monodisperse and size-controlled SCCBs were fabricated by a microfluidic device. Calcination was employed to improve the mechanical stability and lower the fluorescent background of the SCCBs. Immobilization of protein molecules on the surface of the SCCBs through chemical bonds was studied, and the modification condition was optimized to increase the detection sensitivity. Results indicated that the SCCBs as supports were more sensitive (0.92 ng/mL IgG) than the glass beads (27 ng/mL IgG) and the planar carriers (140 ng/mL IgG). A multiplex immunoassay showed the flexibility and feasibility of SCCBs array in clinical applications.
我们开发了一种新型的悬浮阵列用于多重免疫分析，使用二氧化硅胶体晶体珠（SCCB）作为编码载体。通过微流控装置制备了单分散且尺寸可控的SCCB。采用煅烧来提高SCCB的机械稳定性并降低其荧光背景。研究了通过化学键将蛋白质分子固定在SCCB表面的方法，并优化了修饰条件以提高检测灵敏度。结果表明，作为载体的SCCB比玻璃珠（27 ng/mL IgG）和平板载体（140 ng/mL IgG）更灵敏（0.92 ng/mL IgG）。多重免疫分析显示了SCCB阵列在临床应用中的灵活性和可行性。
