Grupo de Biotecnología de Productos Naturales (BIO279), Facultad de Ciencias Experimentales, Universidad de Almería, Campus de La Cañada 04120, Spain.
J Agric Food Chem. 2010 Jan 13;58(1):272-81. doi: 10.1021/jf902970p.
The 11S globulin (legumin) gene family has been characterized in the castor plant Ricinus communis L. Phylogenetic analysis reveals the presence of two diverged subfamilies (RcLEG1 and RcLEG2) comprising a total of nine genes and two putative pseudogenes. The expression of castor legumin genes has been studied, indicating that it is seed specific and developmentally regulated, with a maximum at the stage when cellular endosperm reaches its full expansion (around 40-45 DAP). However, conspicuous differences are appreciated in the expression timing of individual genes. A characterization of the 5'-proximal regulatory regions for two genes, RcLEG1-1 and RcLEG2-1, representative of the two legumin subfamilies, has also been performed by fusion to the GUS reporter gene. The results obtained from heterologous expression in tobacco and transient expression in castor, indicating seed-specific regulation, support the possible utility of these promoters for biotechnological purposes.
蓖麻 11S 球蛋白（legumin）基因家族已在蓖麻植物 Ricinus communis L. 中得到鉴定。系统发育分析显示存在两个分化的亚家族（RcLEG1 和 RcLEG2），共包含 9 个基因和 2 个假定假基因。对蓖麻豆球蛋白基因的表达进行了研究，表明其具有种子特异性和发育调控性，在细胞胚乳达到完全扩张（约 40-45 DAP）时达到最大值。然而，个别基因的表达时间存在明显差异。通过与 GUS 报告基因融合，对两个基因（RcLEG1-1 和 RcLEG2-1）的 5'-近端调控区进行了表征，这两个基因代表了两个球蛋白亚家族。在烟草中的异源表达和在蓖麻中的瞬时表达获得的结果表明种子特异性调控，支持这些启动子在生物技术中的潜在用途。
