Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan.
Anal Chem. 2009 Nov 15;81(22):9274-81. doi: 10.1021/ac901514c.
The combination of laser-induced acoustic desorption and electrospray ionization mass spectrometry (LIAD/ESI/MS) can be used to rapidly characterize chemical compounds separated on a thin layer chromatography (TLC) plate. We performed LIAD analysis by irradiating the rear side of an aluminum-based TLC plate with a pulsed infrared (IR) laser. To efficiently generate and transfer acoustic and shock waves to ablate the analyte-containing TLC gels, a glass slide was attached to the rear of the TLC plate and the gap between the glass slide and the TLC plate was filled with a viscous solution (glycerol). Although the diameter of the laser spot created on the rear of the TLC plate was approximately 0.35 mm, the ablated areas on the front sides of the silica gel bed and the C(18) reverse-phase gel bed had diameters of approximately 1.3 and 3 mm, respectively. The ablated analyte molecules were ionized in an ESI plume and then detected by an ion trap mass analyzer. This TLC/LIAD/ESI/MS approach allowed the components in mixtures of dye standards, drug standards, and rosemary essential oil to be separated and rapidly characterized.
激光诱导声解吸和电喷雾电离质谱（LIAD/ESI/MS）的组合可用于快速分析在薄层色谱（TLC）板上分离的化学化合物。我们通过用脉冲红外（IR）激光辐照基于铝的 TLC 板的背面来进行 LIAD 分析。为了有效地产生和传递声波和冲击波以烧蚀含有分析物的 TLC 凝胶，将玻璃载玻片附着到 TLC 板的背面，并且玻璃载玻片和 TLC 板之间的间隙用粘性溶液（甘油）填充。尽管在 TLC 板背面创建的激光点的直径约为 0.35 毫米，但在硅胶床和 C（18）反相凝胶床的前侧上烧蚀的区域的直径分别约为 1.3 和 3 毫米。烧蚀的分析物分子在 ESI 羽流中被离子化，然后通过离子阱质量分析仪进行检测。这种 TLC/LIAD/ESI/MS 方法允许对染料标准品、药物标准品和迷迭香油混合物中的成分进行分离和快速表征。
