ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, School of Chemistry, University of Wollongong, Wollongong, NSW 2522, Australia.
Prog Lipid Res. 2013 Oct;52(4):329-53. doi: 10.1016/j.plipres.2013.04.005. Epub 2013 Apr 24.
Mass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment; bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-of-the-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates. The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lipid analysis are evaluated.
质谱分析现已成为脂质分析不可或缺的工具，可以说是脂质研究复兴的驱动力。质谱分析以各种形式，独特地能够解析生物系统中脂质广泛的组成和结构多样性。此外，它还能够准确地定量分析与代谢和环境变化相关的脂质群体分子水平的变化，将脂质科学带入“组学”时代。基于质谱的表面分析技术的最新发展正在推动脂质组学领域的进一步扩展。近年来，大量关于脂质的质谱成像的论文证明了这一点。虽然成像质谱提供了新的令人兴奋的可能性，但它只是直接表面分析为脂质研究人员提供的众多机会之一。在这篇综述中，我们描述了直接表面分析脂质的最新技术现状，重点是组织切片、完整细胞和薄层色谱基质。评估了这些不同方法在分析主要脂质类别的适用性及其在脂质分析领域的当前和潜在应用。
