Yu Min, Ma MingXing, Huang Chao, Yang Hui, Lai JianHua, Yan Shan, Li Lin, Xiang MingJun, Tan DeYong
Laboratory of Biochemistry and Molecular Biology, School of Life Science, Yunnan University, Kunming, China.
Cancer Invest. 2009 Dec;27(10):978-83. doi: 10.3109/07357900902769723.
Human arrest-defective-1 (hARD1) was reported to be important in regulating cell cycle and promoting lung cancer cell proliferation. Here we have investigated the correlation between hARD1 and breast cancer. Analysis with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry (FCM) demonstrated that overexpression of hARD1 was associated with increased proliferation of MCF-7 cell, a human breast cancer cell line. Western blotting and immunohistochemical staining assay showed that hARD1 presented higher in breast cancer tissue than the adjacent tissue; accumulation of hARD1 protein was higher in 86% (37/43) of breast cancer, far more than noncancer samples. Our results suggest that hARD1 might play an important role in breast cancer carcinogenesis.
据报道，人类 Arrest-defective-1（hARD1）在调节细胞周期和促进肺癌细胞增殖方面具有重要作用。在此，我们研究了 hARD1 与乳腺癌之间的相关性。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）和流式细胞术（FCM）分析表明，hARD1 的过表达与人类乳腺癌细胞系 MCF-7 细胞增殖增加有关。蛋白质免疫印迹法和免疫组织化学染色分析显示，hARD1 在乳腺癌组织中比相邻组织表达更高；86%（37/43）的乳腺癌中 hARD1 蛋白积累高于非癌样本。我们的结果表明，hARD1 可能在乳腺癌致癌过程中发挥重要作用。
