Sumita Yoshinori, Honda Masaki J, Ueda Minoru, Asahina Izumi, Kagami Hideaki
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Growth Factors. 2010 Feb;28(1):56-65. doi: 10.3109/08977190903373380.
In this study, the effect of growth differentiation factor-5 (GDF-5) on the growth and differentiation of porcine dental papilla- and follicle-derived cells was investigated. Furthermore, the effect was compared with that of BMP-2. Recombinant mouse GDF-5 (rmGDF-5) enhanced alkaline phosphatase (ALP) activity in dental papilla-derived cells in a dose-dependent manner, while ALP activity in dental follicle-derived cells was reduced. In rmGDF-5 stimulated dental papilla-derived cells, the expressions of odontoblast-marker genes were up-regulated. Conversely, recombinant human BMP-2 (rhBMP-2) enhanced ALP activity dose-dependently in both dental papilla- and follicle-derived cells. When combined, GDF-5 did not further enhance BMP-2-induced ALP activities. Rather, GDF-5 reduced BMP-2-induced ALP activities in both dental papilla- and follicle-derived cells. This suggests that affinity of GDF-5 to the shared receptors may be higher than that of BMP-2 in both cell types. These observations indicate that GDF-5 regulates differentiation of both dental papilla and follicle during odontogenesis, co-operatively with other growth factors such as BMP-2.
在本研究中，研究了生长分化因子-5（GDF-5）对猪牙乳头和毛囊来源细胞生长及分化的影响。此外，将该影响与骨形态发生蛋白-2（BMP-2）的影响进行了比较。重组小鼠GDF-5（rmGDF-5）以剂量依赖性方式增强牙乳头来源细胞中的碱性磷酸酶（ALP）活性，而牙囊来源细胞中的ALP活性则降低。在rmGDF-5刺激的牙乳头来源细胞中，成牙本质细胞标记基因的表达上调。相反，重组人BMP-2（rhBMP-2）在牙乳头和牙囊来源细胞中均以剂量依赖性方式增强ALP活性。当两者联合使用时，GDF-5并未进一步增强BMP-2诱导的ALP活性。相反，GDF-5降低了牙乳头和牙囊来源细胞中BMP-2诱导的ALP活性。这表明在两种细胞类型中，GDF-5与共享受体的亲和力可能高于BMP-2。这些观察结果表明，GDF-5在牙齿发育过程中与其他生长因子如BMP-2协同调节牙乳头和牙囊的分化。
