Renzi P M, Du T, Sapienza S, Wang N S, Martin J G
Meakins Christie Laboratories, McGill University, Montreal, Quebec, Canada.
Am Rev Respir Dis. 1991 Feb;143(2):380-5. doi: 10.1164/ajrccm/143.2.380.
We studied the acute effects of interleukin-2 (IL-2), the principal lymphokine responsible for lymphocyte proliferation, on lung mechanics and airway responsiveness to methacholine (MCh) in rats. Lewis (n = 12) and Fisher 344 (n = 13) rats were anesthetized and intubated, and intravenous and intra-arterial lines were inserted. IL-2 (750,000 U/kg) was infused intravenously over 2 to 4 min into seven Lewis and seven Fisher rats, and vehicle alone was administered to five Lewis and six Fisher rats. Blood pressure, heart rate, respiratory frequency (f), tidal volume (VT), minute ventilation (VE), and lung resistance (RL) were measured before and every 5 min for 45 min after the infusion of IL-2. Lung compliance was measured before and 30 min after IL-2. Bronchial provocation testing with MCh was performed 45 min after the infusion of IL-2. Subsequently, the animals were exsanguinated, and the lungs were removed for histologic examination. Infused IL-2 did not alter heart rate or blood pressure, VT, f, VE, and RL increased significantly by 15 min (p less than 0.05), but they returned to baseline by 45 min. Lung compliance decreased significantly in both rat strains. IL-2 increased airway responsiveness only in Lewis rats; the concentration of MCh that caused a doubling of RL (EC200RL) was 0.6 mg/ml and 4.3 mg/ml (p = 0.003) in IL-2-treated and control rats, respectively. The airway responsiveness did not change significantly in Fisher rats; EC200RL was 0.13 and 0.35 mg/ml for IL-2-treated and control rats, respectively (p = 0.09).(ABSTRACT TRUNCATED AT 250 WORDS)
我们研究了白细胞介素-2（IL-2，负责淋巴细胞增殖的主要淋巴因子）对大鼠肺力学及气道对乙酰甲胆碱（MCh）反应性的急性影响。将12只Lewis大鼠和13只Fisher 344大鼠麻醉并插管，插入静脉和动脉导管。将7只Lewis大鼠和7只Fisher大鼠在2至4分钟内静脉输注IL-2（750,000 U/kg），5只Lewis大鼠和6只Fisher大鼠仅给予赋形剂。在输注IL-2前及输注后每5分钟测量45分钟的血压、心率、呼吸频率（f）、潮气量（VT）、分钟通气量（VE）和肺阻力（RL）。在IL-2输注前及输注后30分钟测量肺顺应性。在输注IL-2后45分钟进行MCh支气管激发试验。随后，处死动物，取出肺进行组织学检查。输注的IL-2未改变心率或血压，VT、f、VE和RL在15分钟时显著增加（p<0.05），但在45分钟时恢复至基线。两种大鼠品系的肺顺应性均显著降低。IL-2仅增加Lewis大鼠的气道反应性；在IL-2处理组和对照组大鼠中，使RL加倍的MCh浓度（EC200RL）分别为0.6 mg/ml和4.3 mg/ml（p = 0.003）。Fisher大鼠的气道反应性无显著变化；IL-2处理组和对照组大鼠的EC200RL分别为0.13和0.35 mg/ml（p = 0.09）。（摘要截断于250字）
