MMP-9 基因 c.1562C>T 多态性与 2 型糖尿病大血管病变相关。

Genetic polymorphism c.1562C>T of the MMP-9 is associated with macroangiopathy in type 2 diabetes mellitus.

机构信息

Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, 23 Post Rd Nangang Region, Heilongjiang, Harbin 150001, China.

出版信息

Biochem Biophys Res Commun. 2010 Jan 1;391(1):113-7. doi: 10.1016/j.bbrc.2009.11.012. Epub 2009 Nov 10.

DOI:10.1016/j.bbrc.2009.11.012

PMID:19909726

Abstract

OBJECTIVE

To determine whether the matrix metalloproteinase-9 (MMP-9) c.1562C>T polymorphism has an effect on the plasma MMP-9 levels and the macroangiopathic complications in type 2 diabetes mellitus (T2DM).

METHODS

The genotypes and allelic frequencies of the MMP-9 c.1562C>T were examined with polymerase chain reaction and restriction fragment length polymorphism in 320 patients with T2DM and 160 unrelated healthy subjects. The plasma concentrations of MMP-9 were determined in all subjects.

RESULTS

The mean plasma concentrations of MMP-9 of patients with T2DM were significantly higher than that of controls and the plasma levels of MMP-9 were higher in diabetic patients with macroangiopathy than in patients without macroangiopathy (P<0.05). The genotype (CC, CT, and TT) distribution of c.1562C>T polymorphism of the MMP-9 gene was 60.0%, 31.3%, and 8.8% in diabetic patients with macroangiopathy, 76.3%, 21.3%, and 2.5% in patients without macroangiopathy, and 77.5%, 21.3%, 1.3% in controls, respectively, a significant difference was found between diabetic patients with and without macroangiopathy (P<0.05). The frequency of the allele T was higher in patients with macroangiopathy than in patients without macroangiopathy (24.4% vs 13.1%; P<0.05). Moreover, the plasma MMP-9 levels were markedly higher in patients with TT genotype than those with CC or CT genotype in patients with macroangiopathy (P<0.05).

CONCLUSION

The MMP-9 c.1562C>T gene polymorphism associated with a predisposition to increased plasma MMP-9 levels could constitute a useful predictive marker for diabetic macroangiopathy.

摘要

目的

确定基质金属蛋白酶-9（MMP-9）c.1562C>T 多态性是否对 2 型糖尿病（T2DM）患者的血浆 MMP-9 水平和大血管并发症有影响。

方法

采用聚合酶链反应和限制性片段长度多态性技术，对 320 例 T2DM 患者和 160 例无关健康对照者的 MMP-9 c.1562C>T 基因型和等位基因频率进行检测。所有受试者均测定血浆 MMP-9 浓度。

结果

T2DM 患者的平均血浆 MMP-9 浓度明显高于对照组，且大血管病变患者的血浆 MMP-9 水平高于无大血管病变患者（P<0.05）。MMP-9 基因 c.1562C>T 多态性的基因型（CC、CT 和 TT）分布在大血管病变患者中分别为 60.0%、31.3%和 8.8%，无大血管病变患者中分别为 76.3%、21.3%和 2.5%，对照组中分别为 77.5%、21.3%和 1.3%，大血管病变患者与无大血管病变患者之间存在显著差异（P<0.05）。大血管病变患者中 T 等位基因的频率高于无大血管病变患者（24.4%比 13.1%；P<0.05）。此外，在大血管病变患者中，TT 基因型患者的血浆 MMP-9 水平明显高于 CC 或 CT 基因型患者（P<0.05）。