Division of Health Sciences, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
Brain Res. 2010 Jan 22;1311:158-65. doi: 10.1016/j.brainres.2009.10.074. Epub 2009 Nov 10.
The present study demonstrates changes in rat brain glial metabolism during the acute phase of epilepsy. Status epilepticus (SE) was induced using the lithium-pilocarpine model. Glial metabolism was measured with (14)C-acetate. Local cerebral blood flow and glucose metabolism were also measured using (14)C-N-isopropyl-p-iodoamphetamine (IMP) and (14)C-2-deoxyglucose (2DG), respectively. At the initiation of the seizure, (14)C-acetate uptake did not change significantly. However, a marked increase was observed 2 h after the pilocarpine injection in all brain regions studied. The increase of brain uptake was transient, and the maximum enhancement was seen at 2 h after the pilocarpine injection. The increase of (14)C-acetate uptake was almost to the same degree in all regions, whereas (14)C-IMP and (14)C-2DG uptakes showed a heterogeneous increase. In the case of (14)C-IMP, the highest increase was observed in the thalamus (280%), and a moderate increase (120 to 150%) was seen in the orbital cortex, cingulate cortex and pyriform cortex. (14)C-2DG uptake increased by 130 to 240% in most regions of the brain, however, an increase of only 40 and 20% was observed in the cerebellum and pons-medulla, respectively. These results demonstrated that glial energy metabolism was markedly enhanced during a prolonged seizure. To our knowledge, this study is the first observation showing large and widespread glial metabolic increases in the rat brain during status epilepticus.
本研究在癫痫急性发作期间展示了大鼠脑胶质代谢的变化。癫痫持续状态（SE）通过锂-匹罗卡品模型诱导。使用（14）C-乙酸盐测量胶质代谢。局部脑血流和葡萄糖代谢也分别使用（14）C-N-异丙基-p-碘苯丙氨酸（IMP）和（14）C-2-脱氧葡萄糖（2DG）进行测量。在癫痫发作开始时，（14）C-乙酸盐摄取没有明显变化。然而，在匹罗卡品注射后 2 小时，在所有研究的脑区观察到明显增加。脑摄取的增加是短暂的，最大增强发生在匹罗卡品注射后 2 小时。（14）C-乙酸盐摄取的增加在所有区域几乎达到相同程度，而（14）C-IMP 和（14）C-2DG 摄取表现出异质增加。在（14）C-IMP 的情况下，丘脑观察到最高增加（280%），眶皮质、扣带回和梨状皮质观察到中度增加（120%至 150%）。（14）C-2DG 摄取在大脑的大多数区域增加了 130%至 240%，然而，小脑和脑桥-延髓仅观察到 40%和 20%的增加。这些结果表明，胶质能量代谢在长时间癫痫发作期间明显增强。据我们所知，这项研究首次观察到在癫痫持续状态期间大鼠大脑中广泛而广泛的胶质代谢增加。
