Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK.
J Immunol Methods. 2010 Jan 31;352(1-2):195-7. doi: 10.1016/j.jim.2009.11.003. Epub 2009 Nov 10.
To conserve full complement activity, a number of precautions need to be taken in preparing serum. This is particularly the case with mouse complement where the classical pathway is notoriously unstable.
为了保持补体的完全活性,在制备血清时需要采取一些预防措施。对于小鼠补体来说,情况更是如此,因为经典途径非常不稳定。