Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Campus Universitário, Lagoa Nova, Natal 59072-970, RN, Brazil.
Ecotoxicol Environ Saf. 2010 Mar;73(3):320-5. doi: 10.1016/j.ecoenv.2009.10.008. Epub 2009 Nov 11.
The aim of this study was to access the genotoxic potential of Extremoz Lake waters in Northeastern Brazilian coast, using the Allium cepa system, piscine micronucleus test and comet assay. In addition, heavy metal levels were quantified by atomic absorption flame spectrometry. The results of the A. cepa system showed significant changes in the frequency of chromosome aberrations and in the mitotic index compared to negative control. No significant changes were observed in micronuclei frequency in the erythrocytes of Oreochromis niloticus. The comet assay showed a statistically significant alteration in the level of DNA breaks of O. niloticus. Chemical analysis detected an increase in heavy metal levels in different sampling periods. These results point out a state of deterioration of water quality at Extremoz Lake, caused by heavy metal contamination and genotoxic activity. It is recommended to establish a monitoring program for the presence of genotoxic agents in this water lake.
本研究旨在利用洋葱根尖细胞染色体畸变试验、鱼类微核试验和彗星试验评估巴西东北部沿海极端湖的遗传毒性。此外，还采用原子吸收火焰光谱法测定了重金属含量。结果表明，与阴性对照组相比，洋葱根尖细胞染色体畸变频率和有丝分裂指数显著改变；尼罗罗非鱼红细胞微核频率无显著变化。彗星试验表明，尼罗罗非鱼 DNA 断裂水平发生了统计学上的显著改变。化学分析检测到不同采样期重金属水平升高。这些结果表明极端湖水质恶化，这是由重金属污染和遗传毒性活性引起的。建议建立一个监测计划，以监测该湖水是否存在遗传毒性物质。
