Department of Reproductive and Development Science, University of Trieste, and Children Hospital IRCCS 'Burlo Garofolo' Trieste, Italy.
Clin Exp Immunol. 2010 Feb;159(2):217-23. doi: 10.1111/j.1365-2249.2009.04054.x. Epub 2009 Nov 12.
Anti-transglutaminase antibodies are the diagnostic markers of coeliac disease. A role is suggested for infectious agents in the production of anti-transglutaminase antibodies. The aim was to measure positive anti-transglutaminase antibody levels in children with infectious diseases and to compare immunological and biological characteristics of the anti-transglutaminase antibodies derived from these children with that from coeliac patients. Two hundred and twenty-two children suffering from infectious diseases were enrolled prospectively along with seven biopsy-proven coeliacs. Serum samples were tested for anti-transglutaminase antibodies and anti-endomysium antibodies; positive samples were tested for coeliac-related human leucocyte antigen (HLA)-DQ2/8 and anti-viral antibodies. Purified anti-transglutaminase antibodies from the two study groups were tested for urea-dependent avidity, and their ability to induce cytoskeletal rearrangement and to modulate cell-cycle in Caco-2 cells, using phalloidin staining and bromodeoxyuridine incorporation assays, respectively. Nine of 222 children (4%) tested positive to anti-transglutaminase, one of whom also tested positive for anti-endomysium antibodies. This patient was positive for HLA-DQ2 and was diagnosed as coeliac following intestinal biopsy. Of the eight remaining children, two were positive for HLA-DQ8. Levels of anti-transglutaminase returned to normal in all subjects, despite a gluten-containing diet. Purified anti-transglutaminase of the two study groups induced actin rearrangements and cell-cycle progression. During an infectious disease, anti-transglutaminase antibodies can be produced temporarily and independently of gluten. The infection-triggered anti-transglutaminase antibodies have the same biological properties as that of the coeliacs, with the same in-vivo potential for damage.
抗转谷氨酰胺酶抗体是乳糜泻的诊断标志物。有研究提示感染因子在抗转谷氨酰胺酶抗体的产生中起作用。本研究旨在检测感染性疾病患儿中抗转谷氨酰胺酶抗体的阳性水平，并比较这些患儿与乳糜泻患者来源的抗转谷氨酰胺酶抗体的免疫学和生物学特征。前瞻性纳入 222 例感染性疾病患儿和 7 例经活检证实的乳糜泻患者。检测血清抗转谷氨酰胺酶抗体和抗内膜抗体；阳性样本检测乳糜泻相关人类白细胞抗原（HLA）-DQ2/8 和抗病毒抗体。用尿素依赖性亲合力试验检测来自两组研究对象的纯化抗转谷氨酰胺酶抗体，并分别用鬼笔环肽染色和溴脱氧尿苷掺入试验检测其诱导细胞骨架重排和调节 Caco-2 细胞周期的能力。222 例患儿中有 9 例（4%）抗转谷氨酰胺酶抗体阳性，其中 1 例抗内膜抗体也阳性。该患儿 HLA-DQ2 阳性，经肠道活检后诊断为乳糜泻。其余 8 例患儿中，有 2 例 HLA-DQ8 阳性。尽管患儿摄入含麸质饮食，但所有患儿的抗转谷氨酰胺酶抗体水平均恢复正常。来自两组研究对象的纯化抗转谷氨酰胺酶均能诱导肌动蛋白重排和细胞周期进程。在感染性疾病期间，抗转谷氨酰胺酶抗体可暂时产生，且与麸质无关。感染触发的抗转谷氨酰胺酶抗体与乳糜泻患者的抗体具有相同的生物学特性，在体内具有相同的损伤潜能。
