Sáenz-López P, Gouttefangeas C, Hennenlotter J, Concha A, Maleno I, Ruiz-Cabello F, Cózar J M, Tallada M, Stenzl A, Rammensee H-G, Garrido F, Cabrera T
Servicio de Análisis Clínicos, Hospital Universitario Virgen de las Nieves, Granada, Spain.
Tissue Antigens. 2010 Feb;75(2):110-8. doi: 10.1111/j.1399-0039.2009.01409.x. Epub 2009 Nov 13.
A total of 93 frozen primary renal cell carcinoma (RCC) samples and 31 frozen samples of corresponding normal renal tissue were analyzed for human leukocyte antigen (HLA) class I and HLA-DR expression. Unexpectedly, HLA class I expression was much higher on RCC cells than on normal renal tubular cells. Immunohistochemistry analysis of frozen and paraffin-embedded tissue samples, applying an extended panel of specific anti-HLA monoclonal antibodies, showed elevated HLA class I antigen expression in 95.6% of the tumors vs only 12.9% of normal renal tissues. These findings were confirmed by molecular analysis of HLA heavy chain and beta2-microglobulin (beta2m) transcription levels using quantitative real-time polymerase chain reaction (PCR) on microdissected tissue samples (isolated tumor nests and autologous normal renal tubules) from four patients. These results might help to explain the relatively high success rate of immunotherapy in patients with RCC. The molecular mechanism underlying the increased HLA class I expression in RCC has yet to be elucidated.
对93份原发性肾细胞癌（RCC）冷冻样本和31份相应正常肾组织的冷冻样本进行了人类白细胞抗原（HLA）I类和HLA-DR表达分析。出乎意料的是，RCC细胞上的HLA I类表达比正常肾小管细胞上的表达高得多。对冷冻和石蜡包埋组织样本进行免疫组织化学分析，应用一组扩展的特异性抗HLA单克隆抗体，结果显示95.6%的肿瘤中HLA I类抗原表达升高，而正常肾组织中只有12.9%。通过对来自四名患者的显微切割组织样本（分离的肿瘤巢和自体正常肾小管）进行定量实时聚合酶链反应（PCR），对HLA重链和β2-微球蛋白（β2m）转录水平进行分子分析，证实了这些发现。这些结果可能有助于解释RCC患者免疫治疗相对较高的成功率。RCC中HLA I类表达增加的分子机制尚待阐明。
