Wang Min, Pei Hai-yun, Guan Li-dong, Nan Xue, Bai Ci-xian, Liu Hui, Li Bao-wei, Wang Yun-fang, Pei Xue-tao
Department of Stem Cell and Regenerative Medicine, Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Zhonghua Gan Zang Bing Za Zhi. 2009 Jul;17(7):544-8.
To induce hepatic differentiation of human adipose-derived stem cells (hADSCs) in vitro.
hADSCs were isolated from human adipose tissue and treated with improved hepatic medium containing HGF, bFGF and FGF4. After 7 days of culture, OSM was added to the culture media. Cell growth during hepatic differentiation was evaluated by CCK8 assay. Morphology of differentiation was examined under light microscope. Liver specific genes and proteins were detected by RT-PCR analysis and immunohistochemical staining, respectively. And functional characteristics of hepatocytes were also examined.
The number of hADSCs cultured in the improved hepatic media was increased significantly in comparison to hADSCs cultured in control media from 5 days to 21 days (t=6.59, 8.69, 15.94 and 24.64, respectively, P<0.05). The hADSCs-derived hepatocyte-like cells exhibited hepatocyte morphology, expressed hepatocyte markers, possessed hepatocyte-specific activities, such as uptake and excretion of indocyanine green, glycogen storage and albumin production.
hADSCs can be induced into hepatocyte-like cells in this differentiation system. And this differentiation system promoted the growth of hADSCs.
在体外诱导人脂肪来源干细胞（hADSCs）向肝细胞分化。
从人脂肪组织中分离hADSCs，并用含肝细胞生长因子（HGF）、碱性成纤维细胞生长因子（bFGF）和FGF4的改良肝细胞培养基处理。培养7天后，向培养基中添加制瘤素M（OSM）。通过CCK8法评估肝细胞分化过程中的细胞生长情况。在光学显微镜下检查分化的形态。分别通过逆转录-聚合酶链反应（RT-PCR）分析和免疫组织化学染色检测肝脏特异性基因和蛋白质。并且还检查了肝细胞的功能特性。
与在对照培养基中培养的hADSCs相比，在改良肝细胞培养基中培养的hADSCs数量从第5天到第21天显著增加（t分别为6.59、8.69、15.94和24.64，P<0.05）。hADSCs来源的肝细胞样细胞呈现肝细胞形态，表达肝细胞标志物，具有肝细胞特异性活性，如吲哚菁绿的摄取和排泄、糖原储存和白蛋白产生。
在该分化系统中，hADSCs可被诱导分化为肝细胞样细胞。并且该分化系统促进了hADSCs的生长。
