鼠促肾上腺皮质激素释放激素基因的核苷酸序列和表达。

Nucleotide sequence and expression of the mouse corticotropin-releasing hormone gene.

机构信息

Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.

出版信息

Mol Cell Neurosci. 1991 Jun;2(3):266-73. doi: 10.1016/1044-7431(91)90054-r.

DOI:10.1016/1044-7431(91)90054-r

PMID:19912808

Abstract

The mouse corticotropin-releasing hormone (CRH) gene has been isolated and characterized by DNA sequence analysis. The gene exhibits a structural organization similar to that of the human, rat, and ovine genes with two exons and an intervening sequence of 675 base pairs interrupting the 5' untranslated sequence of the mature mRNA. Analysis of the nucleotide sequence reveals that the mouse CRH peptide is identical at the amino acid level to the human and rat CRH peptides, with only three nucleotide changes between the rat and mouse sequences within the CRH peptide-encoding region. Additionally, the mouse CRH gene exhibits greater than 92% homology to the rat, human, and ovine CRH genes within the first 336 nucleotides of 5' flanking DNA, suggesting that this sequence contains important transcriptional control elements which have been conserved across species to mediate the regulation of this important neuroendocrine peptide. The expression of the mouse CRH gene in brain is demonstrated using in situ hybridization analysis. Mouse CRH mRNA can be detected in the paraventricular nucleus of the hypothalamus and inferior olivary nucleus of mouse brain.

摘要

已通过 DNA 序列分析分离并鉴定了小鼠促肾上腺皮质激素释放激素 (CRH) 基因。该基因的结构组织类似于人类、大鼠和绵羊基因，具有两个外显子和一个 675 个碱基对的间隔序列，中断成熟 mRNA 的 5'非翻译序列。核苷酸序列分析表明，小鼠 CRH 肽在氨基酸水平上与人和大鼠 CRH 肽相同，在 CRH 肽编码区域内，大鼠和小鼠序列之间只有三个核苷酸变化。此外，小鼠 CRH 基因在 5'侧翼 DNA 的前 336 个核苷酸内与大鼠、人类和绵羊 CRH 基因的同源性大于 92%，这表明该序列包含重要的转录调控元件，这些元件在物种间被保守以调节这种重要的神经内分泌肽。通过原位杂交分析证明了小鼠 CRH 基因在大脑中的表达。可以在小鼠大脑的下丘脑室旁核和下橄榄核中检测到小鼠 CRH mRNA。