白细胞介素-1β在体外刺激大鼠肾上腺皮质释放促肾上腺皮质激素释放激素（CRH）和促肾上腺皮质激素（ACTH）。
Interleukin-1beta Stimulates Corticotropin-Releasing Hormone (CRH) and Adrenocorticotropin (ACTH) Release by Rat Adrenal Gland in Vitro.
机构信息
Department of Anatomy, University of Padua, Via Gabelli 65, I-35121 Padua, Italy; and Department of Histology and Embryology, University of Poznan, Swiecickiego 6, PL-60781 Poznan, Poland.
出版信息
Mol Cell Neurosci. 1993 Jun;4(3):267-70. doi: 10.1006/mcne.1993.1034.
CRH and ACTH immunoreactivities (ir) were present in rat adrenal glands but not in adrenocortical autotransplants lacking chromaffin cells. Interleukin-1beta (IL-1beta) dose-dependently elicited CRH-ir and ACTH-ir release by decorticated adrenal fragments mainly composed of zona-medullaris tissue; the minimum effective concentration was 10(-10)/10(-8)M, and the maximal one was 10(-6)M. The IL-1beta (10(-6)M)-induced ACTH release by our preparations was completely blocked by alpha-helical-CRH (10(-6)M), a competitive inhibitor of CRH. These findings suggest that chromaffin medullary cells of the rat adrenals contain a CRH/ACTH system, duplicating that operating at the hypothalamohypophyseal level, which is stimulated by IL-1beta. Thus, the mechanism underlying the well-known glucocorticoid secretagogue effect of interleukins may involve the activation of both the central and the peripheral branch of the hypothalamohypophyseal-adrenal axis.
CRH 和 ACTH 免疫反应（ir）存在于大鼠肾上腺中，但不存在于缺乏嗜铬细胞的肾上腺自体移植中。白细胞介素-1β（IL-1β）以剂量依赖的方式刺激去皮质的肾上腺碎片释放 CRH-ir 和 ACTH-ir，这些碎片主要由束状带组织组成；最小有效浓度为 10（-10）/10（-8）M，最大浓度为 10（-6）M。我们的制剂中，IL-1β（10（-6）M）诱导的 ACTH 释放被 α-螺旋 CRH（10（-6）M）完全阻断，这是 CRH 的竞争性抑制剂。这些发现表明，大鼠肾上腺的嗜铬髓质细胞含有 CRH/ACTH 系统，复制了在下丘脑-垂体水平起作用的系统，该系统受到白细胞介素的刺激。因此，白细胞介素众所周知的糖皮质激素分泌作用的机制可能涉及下丘脑-垂体-肾上腺轴的中枢和外周分支的激活。
Zotero 插件