Deutsches Krebsforschungszentrum, Division of Functional Genome Analysis (B070), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
Infect Genet Evol. 2010 Mar;10(2):229-37. doi: 10.1016/j.meegid.2009.11.003. Epub 2009 Nov 11.
Trypanosoma brucei subspecies undergo establishment and maturation in tsetse flies mid-gut and salivary glands, respectively. Successful establishment of trypanosomes in tsetse mid-gut as well as their migration to saliva gland depends on the ability of these parasites to adapt rapidly to new environmental conditions and to negotiate the physical barriers. To identify subspecies specific genes which are differentially regulated during the establishment of T. brucei subspecies in tsetse flies mid-gut, a comparative genomic analysis between different T. brucei subspecies was performed using microarrays containing about 23040 T. brucei shotgun fragments. The whole genome analyses of RNA expression profiles revealed about 274 significantly differentially expressed genes between T. brucei subspecies. About 7% of the differentially regulated clones did not match to any T. brucei predicted genes. Most of the differentially regulated transcripts are involved in transport across cell membrane and also in the purines metabolism. The genes selectively up regulated in T. brucei gambiense and T. brucei rhodesiense (human infective T. brucei) like snoRNA and HSP70 are expressed in response to stress. The high failure rate in the process of establishment and maturation of T. brucei gambiense during cyclical transmission in tsetse flies may result from the incapacity of this parasite to regulate its growth due to the expression of a variety of chaperones or heat shock proteins. Genes selectively up regulated in T. brucei brucei like NT8.1 nucleoside/nucleobase transporters and S-adenosylmethionine synthetase may favour the establishment of this subspecies in tsetse mid-gut. These genes appear as potential targets for investigations on the development of vaccine blocking the transmission of trypanosomes in tsetse flies.
布氏锥虫亚种分别在采采蝇的中肠和唾液腺中建立和成熟。锥虫在采采蝇中肠的成功建立以及它们向唾液腺的迁移取决于这些寄生虫迅速适应新环境条件和通过物理屏障的能力。为了鉴定在布氏锥虫亚种在采采蝇中肠建立过程中差异表达的亚种特异性基因，使用包含约 23040 个布氏锥虫随机片段的微阵列对不同布氏锥虫亚种进行了比较基因组分析。RNA 表达谱的全基因组分析显示，在布氏锥虫亚种之间有大约 274 个显著差异表达的基因。大约 7%的差异调节克隆与任何布氏锥预测基因都不匹配。大多数差异调节的转录本参与跨细胞膜的运输，也参与嘌呤代谢。在冈比亚锥虫和罗得西亚锥虫（感染人类的布氏锥虫）中选择性上调的基因，如 snoRNA 和 HSP70，是对压力的反应。冈比亚锥虫在采采蝇中周期性传播过程中建立和成熟的高失败率可能是由于寄生虫由于表达各种伴侣蛋白或热休克蛋白而无法调节其生长。在布氏锥虫布鲁斯亚型中选择性上调的基因，如 NT8.1 核苷/碱基转运体和 S-腺苷甲硫氨酸合成酶，可能有利于该亚种在采采蝇中肠的建立。这些基因似乎是研究疫苗开发的潜在靶点，该疫苗可阻断采采蝇传播中的锥虫传播。
