Laboratory of Veterinary Physiology, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Wilrijk, Belgium.
Theriogenology. 2010 Apr 1;73(6):740-7. doi: 10.1016/j.theriogenology.2009.10.002. Epub 2009 Nov 12.
Nowadays, in vitro study of follicular dynamics of primordial and primary follicular stages is limited because in vitro culture systems for these follicles are lacking, both in domestic animal species and in human. Therefore, additional insights might be generated by grafting ovarian tissue into immunodeficient mice to study activation and maturation of early follicular stages. A considerable amount of data has already been gathered in laboratory animals and through clinical application of human assisted reproduction technologies where live births were reported recently after the use of (cryopreserved) ovarian grafts. However, given that human preantral follicles are difficult to obtain and that there are many similarities between the bovine and human species with regard to ovarian physiology, the bovine model offers exciting additional prospects and is therefore discussed in more detail. This review will focus on recent developments related to preantral follicle and (repeated) ovarian tissue retrieval and xenotransplantation of (bovine) ovarian tissue strips to immunodeficient mice as a model to study preantral follicular dynamics. Different grafting strategies will be discussed as well as the consequences of this procedure on the viability and dynamic behavior of the grafted tissue and follicles.
如今，由于缺乏针对原始卵泡和初级卵泡阶段的体外培养系统，因此对于这些卵泡的体外研究受到限制，无论是在家畜物种还是在人类中都是如此。因此，通过将卵巢组织移植到免疫缺陷小鼠中，可以进一步研究早期卵泡阶段的激活和成熟。在实验室动物和人类辅助生殖技术的临床应用中已经积累了相当多的数据，最近在使用（冷冻保存的）卵巢移植物后报告了活产。然而，鉴于人类窦前卵泡难以获得，并且在卵巢生理学方面牛和人类之间有许多相似之处，因此牛模型提供了令人兴奋的额外前景，并因此进行了更详细的讨论。这篇综述将重点介绍与窦前卵泡和（重复）卵巢组织采集以及将（牛）卵巢组织条异种移植到免疫缺陷小鼠中作为研究窦前卵泡动态的模型相关的最新进展。还将讨论不同的移植策略，以及该程序对移植物和卵泡的存活和动态行为的影响。
