KCNH-2 和 KCNE-2 在 I(Kr) 通道形成中的化学计量关系。

The stoichiometric relationship between KCNH-2 and KCNE-2 in I(Kr) channel formation.

机构信息

Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea.

Korea Institute of Toxicology, 100 Jangdong, Yuseong, Daejeon, 305-343, Republic of Korea; Pharmacology and Toxicology, School of Engineering, University of Science and Technology, 113 Gwahangno, Yuseong, Daejeon, 305-333, Republic of Korea.

出版信息

Int J Cardiol. 2010 Nov 19;145(2):272-274. doi: 10.1016/j.ijcard.2009.09.552. Epub 2009 Nov 13.

DOI:10.1016/j.ijcard.2009.09.552

PMID:19913309

Abstract

KCNH-2 and KCNE-2 may encode the channel-forming alpha- and regulatory beta-subunits, respectively, of I(Kr) channels, which are involved in inherited or acquired long QT syndrome. However, in contrast to other multimeric channels, the stoichiometry of KCNH-2 and KCNE-2, which should be reasonably maintained if they are to be accepted as the components of a multi-molecular complex, has not been established, yet. In this study, we found that the protein expression of KCNE-2 was adequate to support the formation of a complex with KCNH-2; however, the level of transcription was not. This finding, together with previous data from electrophysiological and molecular biological studies, supports that KCNH-2 and KCNE-2 are molecular components of I(Kr) channels.

摘要

KCNH-2 和 KCNE-2 可能分别编码 I(Kr) 通道的形成性 α-亚基和调节性 β-亚基，该通道与遗传性或获得性长 QT 综合征有关。然而，与其他多聚体通道不同，如果它们被认为是多分子复合物的组成部分，那么 KCNH-2 和 KCNE-2 的比例关系尚未得到确定。在这项研究中，我们发现 KCNE-2 的蛋白表达足以支持与 KCNH-2 形成复合物；然而，转录水平却没有。这一发现，以及以前来自电生理学和分子生物学研究的数据，支持 KCNH-2 和 KCNE-2 是 I(Kr) 通道的分子组成部分。