Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, 3422 Sesoko, Motobu, Okinawa 905-0227, Japan.
Comp Biochem Physiol A Mol Integr Physiol. 2010 Feb;155(2):237-44. doi: 10.1016/j.cbpa.2009.11.004. Epub 2009 Nov 12.
To investigate the role of estrogen in the gonad of yellowtail clownfish Amphiprion clarkii, we isolated cDNA encoding cytochrome P450 aromatase (Cyp19a1a) from the adult ovary. The full-length cDNA of clownfish cyp19a1a is 1928-bp long and encodes 520 amino acids. Real-time quantitative RT-PCR analysis showed that cyp19a1a was expressed mainly in the ovary of female-phase fish. In situ hybridization and immunohistochemical observations showed that positive signals were restricted to the ovarian follicle of the female-phase fish. In contrast, Cyp19a1a signal was not detected in the ambisexual gonad of the male-phase fish. These findings suggest that Cyp19a1a is involved in oogenesis in the female-phase fish, but not in the ambisexual gonad of male-phase fish.
为了研究雌激素在黄尾小丑鱼（Amphiprion clarkii）性腺中的作用，我们从成年卵巢中分离出编码细胞色素 P450 芳香酶（Cyp19a1a）的 cDNA。小丑鱼 cyp19a1a 的全长 cDNA 长 1928bp，编码 520 个氨基酸。实时定量 RT-PCR 分析表明，cyp19a1a 主要在雌性期鱼类的卵巢中表达。原位杂交和免疫组织化学观察表明，阳性信号仅限于雌性期鱼类的卵巢滤泡。相比之下，Cyp19a1a 信号在雄性期鱼类的两性性腺中未被检测到。这些发现表明 Cyp19a1a 参与雌性期鱼类的卵子发生，但不参与雄性期鱼类的两性性腺。
