Institute of Pharmacy, Ernst-Moritz-Arndt-University, Friedrich-Ludwig-Jahn-Strasse 17, D-17487 Greifswald, Germany.
Bioorg Med Chem Lett. 2010 Jan 1;20(1):232-5. doi: 10.1016/j.bmcl.2009.10.125. Epub 2009 Oct 30.
(2R,3S,4R,5R)-5-(6-Amino-9H-purin-9-yl)-3-hydroxy-4-[2-(methylamino)benzamido]tetrahydrofuran-2-yl-methoxy[(hydroxy)phosphoryloxy][(hydroxy)phosphoryl]dichloromethylphosphonic acid was synthesized as a chemically and metabolically stable analog of ATP substituted with a fluorescent methylanthranoyl (MANT) residue. The compound is intended for studying the binding site and function of adenylyl cyclases (ACs), which was exemplified by studying its interaction with Bacillus anthracis edema factor (EF) AC exotoxin.
(2R,3S,4R,5R)-5-(6-氨基-9H-嘌呤-9-基)-3-羟基-4-[2-(甲氨基)苯甲酰胺基]四氢呋喃-2-基甲氧基[[(羟基)膦酰基]氧基][(羟基)膦酰基]二氯甲基膦酸被合成为一种用荧光甲酰基（MANT）残基取代的 ATP 的化学和代谢稳定的类似物。该化合物用于研究腺苷酸环化酶（ACs）的结合位点和功能，这通过研究其与炭疽杆菌水肿因子（EF）AC 外毒素的相互作用得到了例证。
