应用气相色谱/质谱分析尿液样本鉴定马的隐睾症。

Identification of cryptorchidism in horses by analysing urine samples with gas chromatography/mass spectrometry.

机构信息

Racing Laboratory, The Hong Kong Jockey Club, Sha Tin Racecourse, Sha Tin, N.T., Hong Kong, China.

出版信息

Vet J. 2011 Jan;187(1):60-4. doi: 10.1016/j.tvjl.2009.10.010. Epub 2009 Nov 14.

DOI:10.1016/j.tvjl.2009.10.010

PMID:19914849

Abstract

Currently there are two common radioimmunoassay-based methods for the detection of equine cryptorchidism; one measures testosterone concentrations in peripheral blood samples taken before and after an intravenous injection of human chorionic gonadotrophin (hCG) and the other measures plasma estrone sulfate. However, each of these invasive methods has its own shortfalls and neither gives unequivocal results. In this article a highly reliable gas chromatography/mass spectrometry (GC/MS) method is described based on the analysis of urine samples for the identification of cryptorchidism in horses, some as young as 2 years old.

摘要

目前，有两种常用的基于放射免疫分析的方法来检测马的隐睾症；一种方法是测量静脉注射人绒毛膜促性腺激素（hCG）前后外周血样中的睾酮浓度，另一种方法是测量血浆雌酮硫酸盐。然而，这些侵入性方法都有其自身的缺点，而且都没有给出明确的结果。在本文中，描述了一种基于尿液样本分析的高度可靠的气相色谱/质谱（GC/MS）方法，用于鉴定马的隐睾症，其中一些马只有 2 岁。