Jarori G K, Maitra P K
Tata Institute of Fundamental Research, Colaba, Bombay, India.
FEBS Lett. 1991 Jan 28;278(2):247-51. doi: 10.1016/0014-5793(91)80127-o.
Glucose 6-phosphate dehydrogenase catalyzes the oxidation of glucose 6-phosphate, resulting in the formation of 6-phosphogluconolactone. As this compound is unstable, it has not been characterized directly. NMR provides a way to directly monitor all components of a reaction and study their structure. Here we report some results on the glucose 6-phosphate dehydrogenase reaction using 31P and 13C-NMR. Our results indicate that two different lactones, namely gamma (1-4) and delta (1-5) 6-phosphogluconolactones, are formed as products in this reaction. This is in contrast to an earlier suggestion that glucose 6-phosphate dehydrogenase produces only the delta-lactone. On the basis of these results, a new mechanisms for dehydrogenation of the sugar phosphate is proposed.
葡萄糖6-磷酸脱氢酶催化葡萄糖6-磷酸的氧化，生成6-磷酸葡糖酸内酯。由于该化合物不稳定，尚未对其进行直接表征。核磁共振（NMR）提供了一种直接监测反应所有成分并研究其结构的方法。在此，我们报告了使用31P和13C-NMR对葡萄糖6-磷酸脱氢酶反应的一些研究结果。我们的结果表明，两种不同的内酯，即γ（1-4）和δ（1-5）6-磷酸葡糖酸内酯，在该反应中作为产物形成。这与早期认为葡萄糖6-磷酸脱氢酶仅产生δ-内酯的观点相反。基于这些结果，提出了一种新的磷酸糖脱氢机制。
